Surface display of functional proteins in a broad range of gram negative bacteria

ABSTRACT

The present invention relates to a method for the surface display of a recombinant polypeptide on the surface of a host cell, said method comprising the steps: (a) providing a host cell transformed with a nucleic acid fusion operatively linked with an expression control sequence, said nucleic acid fusion comprising: (i) a portion encoding a signal peptide, (ii) a portion encoding the recombinant polypeptide to be displayed, (iii) a portion encoding a transmembrane linker, and (iv) a portion encoding the trans porter domain of an EhaA protein, and (b) culturing the host cell under conditions wherein the nucleic acid fusion is expressed and the expression product comprising the recombinant polypeptide is displayed on the surface of the host cell.

CROSS REFERENCE TO RELATED APPLICATION

This application is a 35 U.S.C. 371 National Phase Entry Application from PCT/EP2014/054383, filed Mar. 6, 2014, which claims the benefit of European Patent Application No. 13159041.6 filed on Mar. 13, 2013, the disclosures of which are incorporated herein in their entirety by reference.

The present invention relates to a method for the surface display of a recombinant polypeptide on the surface of a host cell, said method comprising the steps: (a) providing a host cell transformed with a nucleic acid fusion operatively linked with an expression control sequence, said nucleic acid fusion comprising: (i) a portion encoding a signal peptide, (ii) a portion encoding the recombinant polypeptide to be displayed, (iii) a portion encoding a transmembrane linker, and (iv) a portion encoding the transporter domain of an EhaA protein, and (b) culturing the host cell under conditions wherein the nucleic acid fusion is expressed and the expression product comprising the recombinant polypeptide is displayed on the surface of the host cell.

Expression in bacteria is the method of choice for the commercial production of pharmaceutical and industrial proteins. E. coli is a versatile lab organism for the expression of recombinant proteins, their investigation and in some issues for even their production in preparative scales. For such purposes, a wide variety of vectors e.g. plasmids, suitable mutants and protocols are available. For being used in industrial applications or crude biotechnological production processes E. coli has several disadvantages. It usually does not grow to high cell densities in fermentation processes and needs high concentrations of glucose to gain growth energy. Being a natural gut inhabitant, it is not used to crude environments as water or soil, rather sensible to harsh treatments and is not resistant to organic solvents. Therefore, the aim of the present invention is to combine the advantages of surface display of recombinant proteins with the activa of host organisms different from E. coli, e.g. the natural soil bacterium Pseudomonas (putida), resistant to a variety of organic solvents, organisms that exploit other sources than glucose to gain growth energy e.g. Rhodobacter (light) or Cupriavidus necator (oxyhydrogen) and organisms that are—in contrast to E. coli—suitable for the efficient production of ethanol or other biofuels like Zymomonas. For this purpose an optimized second generation surface display system was developed, the MATE (maximized autotransporter expression) system, shown to be superior in E. coli and proven to be a versatile tool for the surface display of recombinant proteins in a wide variety of gram negative bacteria different from E. coli.

Autotransporter proteins are known to facilitate secretion either through release by E. coli proteases or through self-proteolysis after translocation. The perspectives for commercial protein secretion with the various autotransporter families were reviewed by Jong et al., (2010). Because the passenger remains attached to the cell, autotransporter secretion systems require endoproteolytic cleavage. The natural E. coli outer membrane protease OmpT is known to cleave in the linker region of some autotransporters. The display of the cholera toxin B subunit (CtxB) with AIDAI autotransporter in OmpT positive strains led to the release of the passenger into the growth medium (Maurer 1997, Jose 2002). The release of the passenger naturally lowers the amount of recombinant protein at the surface, reducing the efficiency of whole cell biocatalysis or screening. For this reason, Autodisplay in E. coli has typically been carried out in OmpT negative strains such as UT5600 or BI21.

Several autotransporters have passengers that naturally undergo self-proteolysis, releasing themselves into the growth medium after translocation across the outer membrane. By replacing the natural passenger with the target protein, this system has recently been utilised for secretion of a recombinant passenger (Sevastsyanovich et al. 2012).

Among other systems for the secretion of proteins in Gram-negative bacteria, the autotransporter pathway represents a solution of impressing simplicity. It is possible to transport a protein, regardless whether it is recombinant or the natural passenger, to the actual outer membrane, as long as its coding region lies between a typical signal peptide and a C-terminal domain called β-barrel. Based on these findings the autodisplay system has been developed by the use of the natural E. coli autotransporter protein AIDA-I (the adhesin involved in diffuse adherence) in a homologous E. coli host background (Jose et al. 2007). With the aid of a typical signal peptide, the precursor is transported across the inner membrane. Arrived in the periplasm, the C terminal part of the precursor forms a porin-like structure, a so-called β-barrel, within the outer membrane and through this pore the N terminally attached passenger (the actual protease) is translocated to the cell surface. To obtain full surface exposure of the passenger, a linker peptide is required in between the β-barrel and the passenger.

EhaA is an autotransporter protein derived from E. coli strain O157:H7. It has been demonstrated that EhaA is located at the cell surface and resulted in the formation of large cell aggregates, promoted significant biofilm formation and mediated adhesion to primary epithelial cell of the bovine terminal rectum (Wells et al., 2008).

EhaA has an identity to AIDA-I on the nucleic acid level of about 43% only. On level of the amino acid sequence, the identity is only about 34%.

Up to now, EhaA has not been expressed in a heterologous cell. In the present invention, it is has been surprisingly found that the transporter domain of EhaA can be successfully used for surface-display of a recombinant passenger proteins in a host cell heterologous to E. coli. Examples of such heterologous cells are Pseudomonas spp., Rhodobacter spp., Zymomonas spp. and Cupriavidus spp. Surprisingly, the cells expressing a construct of the present invention on the cell surface do not form aggregates or a biofilm. Aggregation or biofilm formation would make such cells unsuitable for biotechnological applications (for example, suspension culture).

By the heterologous expression, autodisplay of recombinant passenger proteins on a bacterial cell surface can be employed in species being more suitable in biotechnological applications than E. coli. Such cells include Pseudomonas spp., Rhodobacter spp., Zymomonas spp. and Cupriavidus spp., but are not limited thereto.

In the present invention, it has surprisingly found that the MATE system described herein results in a higher activity of the passenger displayed on a host cell than the AIDA-I system described in the prior art. For example, an EstA catalytic domain demonstrates an improved activity of conversion of p-nitrophenyl acetate compared with expression by the AIDA-I system (Example 6, FIG. 8). Furthermore, by the MATE system, the EstA catalytic domain can be functionally expressed on the surface of heterologous species such as Salmonella enterica, Pseudomonas putida or Zymomonas mobilis (Examples 7 to 9).

Example 10 describes the functional surface display of an active heterologous endoglucanase obtained from Bacillus subtilis on the ethanologenic bacteria Zymobacter palmae and Zymomonas mobilis using the MATE system of the present invention.

Example 11 describes the functional display of heterologeous bacterial cellulases obtained from different species (an endoglucanase obtained from Bacillus subtilis, an exoglucanase obtained from Clostridium thermocellum and a β-glucosidase obtained from Clostridium thermocellum) on the surface of Pseudomonas putida with the pMATE system of the present invention. Furthermore, it could be demonstrated that these three enzymes obtained from different species expressed on the cell surface together were able to degrade cellulose or a lignocellulosic substrate into reducing sugars (in particular glucose, cellubiose and cellulose-polysaccharide chains of variable length).

A first aspect of the present invention relates to a method for displaying a recombinant polypeptide on the surface of a host cell, said method comprising the steps:

-   -   (a) providing a host cell transformed with a nucleic acid fusion         operatively linked with an expression control sequence, said         nucleic acid fusion comprising:         -   (i) a portion encoding a signal peptide,         -   (ii) a portion encoding the recombinant polypeptide to be             displayed,         -   (iii) a portion encoding a transmembrane linker, and         -   (iv) a portion encoding the transporter domain of an EhaA             protein, and     -   (b) culturing the host cell under conditions wherein the nucleic         acid fusion is expressed and the expression product comprising         the recombinant polypeptide is displayed on the surface of the         host cell.

The expression system as described herein employing the EhaA autotransporter domain is also termed MATE (maximized autotransporter expression) system of pMATE system. Plasmids (in particular expression plasmids comprising the nucleic acid fusion as described herein) to be used in the MATE system are also termed by the prefix “pMATE”.

The expression product defined in step (b) is also termed herein as “polypeptide fusion”.

By the method of the present invention, a functional recombinant polypeptide can be displayed. The recombinant polypeptide to be displayed may also be termed “passenger”, “passenger polypeptide” or “passenger protein”.

Step (a) of the methods of the present invention refers to the provision of a host cell. The host cell used in the method of the present invention is preferably a bacterium, more preferably a Gram-negative bacterium.

The Gram-negative bacterium can be selected from E. coli, Salmonella spp., Zymomonas spp., Zymobacter spp., Pseudomonas spp., Cupriavidus spp. (formerly known as Ralstonia spp.), Rhodobacter spp., Acinetobacter spp., Gluconobacter spp., Gluconacetobacter spp., Acidomonas spp., Acetobacter spp., Paracoccous spp., Rhizobium spp., Xanthomonas spp.

The Gram-negative bacterium is preferably not E. coli. In this case, the host cell is heterologous to the transporter domain of the EhaA protein. The Gram-negative bacterium is preferably selected from Salmonella spp., Zymomonas spp., Zymobacter spp., Pseudomonas spp., Cupriavidus spp., Rhodobacter spp., Acinetobacter spp., Gluconobacter spp., Gluconacetobacter spp., Acidomonas spp., Acetobacter spp., Paracoccous spp., Rhizobium spp., and Xanthomonas spp.

Another preferred selection of the Gram-negative bacterium is the selection from Zymomonas spp., Zymobacter spp., Pseudomonas spp., Cupriavidus spp., Rhodobacter spp., Acinetobacter spp., Gluconobacter spp., Gluconacetobacter spp., Acidomonas spp., Acetobacter spp., Paracoccous spp., Rhizobium spp., and Xanthomonas spp.

Another preferred selection of the Gram-negative bacterium is the selection from Pseudomonas spp., Rhodobacter spp., Zymomonas spp. and Cupriavidus spp.

A preferred Salmonella species is Salmonella enterica. A preferred Zymomonas species is Zymomonas mobilis. More preferred is Zymomonas mobilis strain DSM 3580. A preferred Zymobacter species is Zymobacter palmae. A preferred Pseudomonas species is Pseudomonas putida or Pseudomonas fluorescens. A preferred Cupriavidus species is Cupriavidus necator or Cupriavidus metallidurans. A preferred Rhodobacter species is Rhodobacter capsulatus. A preferred Acinetobacter species is Acinetobacter baylyi ADP1. A preferred Gluconobacter species is Gluconobacter oxydans. A preferred Acetobacter species is Acetobacter xylinum. A preferred Paracoccous species is Paracoccous denitrificans. A preferred Rhizobium species is Rhizobium meliloti. A preferred Xanthomonas species is Xanthomonas campestris.

The Gram-negative bacterium can be selected from Salmonella enterica, Zymomonas mobilis Zymobacter palmae, Pseudomonas putida, Pseudomonas fluorescens, Cupriavidus necator, Cupriavidus metallidurans, Rhodobacter capsulatus, Acinetobacter baylyi ADP1, Gluconobacter oxydans, Gluconacetobacter spp., Acidomonas spp., Acetobacter xylinum, Paracoccous denitrificans, Rhizobium meliloti, and Xanthomonas campestris.

The gram-negative bacterium can be an ethanologenic bacterium, for example Zymomonas mobilis, Zymobacter palmae, P. putida or Klebsiella spp.

According to the present invention, a host cell, particularly a host bacterium is provided which is transformed with a nucleic acid fusion operatively linked with an expression control sequence, i.e. a promoter, and optionally further sequences required for gene expression in the respective host cell. The skilled person knows suitable promoters and expression control sequences, in particular for expression in the host cell species as described herein. The promoter or/and the expression control sequence may be homologous or heterologous to the host cell. Preferably, the nucleic acid fusion is located on a recombinant vector, e.g. a plasmid vector.

By the method of the present invention, a combination of enzymes can be expressed. The host cell may be transformed with at least one nucleic acid fusion, for instance two, three, four, five or even more nucleic acid fusions. If two or more nucleic acid fusions are transformed into a host cell, the nucleic acid fusions preferably encode different recombinant polypeptides as described herein. If a host cell transformed with several nucleic acid fusions is used, these nucleic acid fusions may be located on a single vector or on a plurality of vectors.

At least one host cell as described herein, for instance two, three, four, five, six or even more host cells as described herein may be provided in the methods of the present invention. Each of these host cells is transformed with one nucleic acid fusion or at least one nucleic acid fusion, as described herein. Preferably, the nucleic acid fusions transformed in the at least one host cell encode different recombinant polypeptides as described herein.

The different recombinant polypeptides which may be provided in one or at least one host cell may form a functional unit, for instance the subunits of a functional unit, such as the subunits of an enzyme or the subunits or/and components of an enzyme complex.

An example for expression of at least one recombinant passenger is an endoglucanase (EC 3.2.1.4), an exoglucanase (EC 3.2.1.91) or/and a β-glucosidase (EC 3.2.1.21), and any combination thereof, expressed by the method of the present invention, as described herein.

The nucleic acid fusion comprises (i) a portion encoding a signal peptide, preferably a portion coding for a Gram-negative signal peptide allowing for transport into the periplasm through the inner cell membrane. The signal peptide may be a signal peptide homologous to the host cell. The signal peptide may also be a signal peptide heterologous to the host cell. An example of a suitable signal peptide is the CtxB signal peptide. The signal peptide can be cleaved off during maturation of the polypeptide fusion.

Further, the nucleic acid fusion comprises (ii) a portion encoding the recombinant polypeptide to be displayed. Any recombinant polypeptide can be displayed. The Examples of the present invention cover a broad range of recombinant polypeptides including GFP, RFP, an esterase EstA, an endoglucanase, an exoglucanase, a β-glucosidase and a polypeptide as short as His₆ (HHHHHH) displayed by the nucleic acid fusion of the present invention on the surface of a host cell.

In particular, the passenger is an enzyme, for example an enzyme selected from endoglucanases (EC 3.2.1.4), exoglucanases (EC 3.2.1.91), β-glucosidases (EC 3.2.1.21). ligninases, lignin peroxidases (EC 1.11.1.14), lipases (EC 3.1.1.3), esterases (EC 3.1.1.1), peroxidases (1.11.1.16), laccases (1.10.3.2), cellobiose dehydrogenases (EC 1.1.99.18), hexose-isomerases, pentose-isomerases, cytochrome P450 enzymes, formiat dehydrogenases (EC 1.2.1.2), cytochrome P450 reductase (EC 1.6.2.4), NADH oxidases (EC 1.6.3.x), dehydrogenases, prenyltransferases, xylanases (EC 3.2.1.8), proteases, transfructosidases, transglucosidases amylases (EC 3.2.1.1, EC 3.2.1.2, EC 3.2.1.3, EC 3.2.1.68), aldolases (EC 4.1.2.13), pectinases (EC 3.2.1.15), glucose oxidases (EC 1.1.3.4), hexose oxidases (EC 1.1.3.5), lactose oxidases (EC 1.1.3.x), sorbitol/xylitol oxidases (EC 1.1.3.41), D-gluconolactone oxidases (EC 1.1.3.x), pyranose oxidases (EC 1.1.3.10), isoamyl alcohol oxidases (EC 1.1.3.x), long-chain alcohol oxidases (EC 1.1.3.20), vanillyl-alcohol oxidases (EC 1.1.3.38), aryl-alcohol oxidases (EC 1.1.37), D-amino acid oxidases (EC 1.4.3.1, EC 1.4.3.3, EC 1.4.3.19), L-amino acid oxidases (EC 1.4.3.2, EC 1.4.3.1.1, EC 1.4.3.14, EC 1.4.3.16), monoamine oxidases (EC 1.4.3.4), fructosyl amine oxidases (EC 1.5.4.x), sulfhydril oxidases (EC 1.8.3.2), aromatic hydrocarbon dioxygenases (EC 1.14.12.x), and dehalogenases.

The dehalogenases include haloalkane dehalogenases, halocarboxylic acid dehalogenases, halohydrin dehalogenases, chloroacrylic acid dehalogenases, hexachlorocyclohexane dechlorinases, and atrazine chlorohydrolases, but are not limited thereto.

In a preferred embodiment an endoglucanase (EC 3.2.1.4), an exoglucanase (EC 3.2.1.91) or/and a β-glucosidase (EC 3.2.1.21), or any combination thereof, is expressed by the method of the present invention. If a combination of two or three of these enzymes is expressed, these enzymes can be co-expressed in a common cell, or each enzyme can be expressed in a separate cell. A combination of an endoglucanase (EC 3.2.1.4), an exoglucanase (EC 3.2.1.91) or/and a β-glucosidase (EC 3.2.1.21), expressed by the method of the present invention can by used in cellulose or/and lignocellulose degradation.

A preferred exoglucanase of the present invention can be obtained from Clostridium thermocellum.

Another preferred exoglucanase comprises amino acid positions 38-831 of SEQ ID NO:21, or a sequence being at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% identical to positions 38-831 of SEQ ID NO:21.

A preferred exoglucanase of the present invention is encoded by SEQ ID NO:20, in particular by positions 112-2493 of SEQ ID NO:20, or by a sequence being at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% identical to positions 112-2493 of SEQ ID NO:20.

A preferred endoglucanase of the present invention can be obtained from Bacillus subtilis.

A preferred endoglucanase of the present invention comprises amino acid positions 38-511 of SEQ ID NO:23, or a sequence being at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% identical to positions 38-511 of SEQ ID NO:23.

A preferred endoglucanase of the present invention is encoded by SEQ ID NO:22, in particular by positions 112-1533 of SEQ ID NO:22, or by a sequence being at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% identical to positions 112-1533 of SEQ ID NO:22.

A preferred β-glucosidase of the present invention can be obtained from Clostridium thermocellum.

A preferred β-glucosidase of the present invention comprises amino acid positions 38-488 of SEQ ID NO:25, or a sequence being at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% identical to positions 38-488 of SEQ ID NO:25.

A preferred β-glucosidase of the present invention is encoded by SEQ ID NO:24, in particular by positions 112-1464 of SEQ ID NO:24, or by a sequence being at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% identical to positions 112-1464 of SEQ ID NO:24.

In another preferred embodiment, the passenger is an esterase. Esterases represent a group of hydrolases that have a wide substrate tolerance and are able to catalyze a broad spectrum of reactions even in organic solvents. Moreover, esterases show high regio- and/or enantioselectivity. They are not restricted to dissolving an ester bond, but can also catalyze its formation and usually do not require any cofactors. Therefore, esterases in general are attractive tools for industrial applications such as chiral synthesis of pharmaceuticals or agrochemicals. Therefore lipolytic enzymes like esterases are attractive biotechnological tools. Another branch of lypolytic enzymes, named lipases (triacylglycerol acylhydrolases EC 3.1.1.3), which catalyze the hydrolysis of triglycerides in aqueous media, liberating free fatty acids and glycerol, or the reverse reaction in organic solvents as well, have gained particular interest, since they simultaneously show in addition to high enantio- and/or regio-selectivity an increased catalytic activity and thermostability in organic solvents. Contrary to esterases, which preferentially break ester bonds of short chain fatty acids, lipases are able to catalyze the hydrolysis of water-insoluble long-chain acylglycerols. So far, lipases have been established in numerous industries, such as the food industry, paper manufacturing, pharmaceutical processing, and detergents industry, reflecting their great importance.

Furthermore, the nucleic acid fusion comprises (iii) a portion encoding a transmembrane linker which is required for the presentation of the passenger polypeptide (ii) on the outer surface of the outer membrane of the host cell. A transmembrane linker domain may be used which is homologous with regard to the autotransporter, i.e. the transmembrane linker domain is encoded by a nucleic acid portion directly 5′ to the autotransporter domain in the EhaA protein. Also a transmembrane linker domain may be used which is heterologous with regard to the autotransporter. The length of the transmembrane linker is preferably 30-160 amino acids. The transmembrane linker is preferably a transmembrane linker obtained from an EhaA protein as described herein.

Further, the nucleic acid fusion comprises (iv) a transporter domain of an EhaA protein. In the present invention, autodisplay by the transporter domain of an EhaA protein is the recombinant surface display of proteins or polypeptides by means of the autotransporter in any Gram-negative bacterium. The transporter domain of the EhaA protein according to the invention is preferably capable of forming a β-barrel structure.

The transporter domain of EhaA as described herein include variants which can e.g. be obtained by altering the amino acid sequence in the loop structures of the β-barrel not participating in the transmembrane portions. Optionally, the nucleic acid portions coding for the surface loops can be deleted completely. Also within the amphipathic β-sheet conserved amino exchanges, i.e. the exchange of an hydrophilic by another hydrophilic amino acid or/and the exchange of a hydrophobic by another hydrophobic amino acid may take place. Preferably, a variant has a sequence identity of at least 70%, at least 90%, at least 95% or at least 98% on the amino acid level to the respective native sequence of the autotransporter domain, in particular in the range of the β-sheets.

The EhaA protein of the present invention and a nucleic acid encoding therefor can be obtained from E. coli. The amino acid sequence of the E. coli EhaA protein is in particular described by YP_003498036 (for example, Version YP_003498036.1, genbank identifier GI:291281218). This amino acid sequence is described by SEQ ID NO:15. As can be seen from FIG. 25, the identity of EhaA and AIDA-I is about 43% on the nucleic acid level and about 34% on the amino acid.

The transmembrane linker (ii) is in particular encoded by a sequence comprising a sequence selected from the group consisting of:

-   -   (a) a nucleotide sequence comprising SEQ ID NO:16,     -   (b) a nucleotide sequence encoding SEQ ID NO:17,     -   (c) nucleotide sequences comprising a sequence being at least         70%, at least 80%, at least 90%, at least 95%, at least 98%, or         at least 99% identical to SEQ ID NO:16 or/and a nucleotide         sequence encoding SEQ ID NO:17, and     -   (d) nucleotide sequences which encodes the polypeptides encoded         by (a), (b) or/and (c) within the scope of the degeneracy of the         genetic code.

The transmembrane linker (ii) in particular comprises a sequence selected from the group consisting of:

-   -   (a) an amino acid sequence comprising SEQ ID NO:17, and     -   (b) sequences which are at least 70%, at least 80%, at least         90%, at least 95%, at least 98%, or at least 99% identical to         the sequences of (a).

The transporter domain of the EhaA protein (iii) is in particular encoded by a sequence comprising a sequence selected from the group consisting of:

-   -   (a) a nucleotide sequence comprising SEQ ID NO:18,     -   (b) a nucleotide sequence encoding SEQ ID NO:19,     -   (c) nucleotide sequences comprising a sequence being at least         70%, at least 80%, at least 90%, at least 95%, at least 98%, or         at least 99% identical to SEQ ID NO:18 or/and a nucleotide         sequence encoding SEQ ID NO:19, and     -   (d) nucleotide sequences which encodes the polypeptides encoded         by (a), (b) or/and (c) within the scope of the degeneracy of the         genetic code.

The transporter domain of the EhaA protein (iii) in particular comprises a sequence selected from the group consisting of:

-   -   (a) an amino acid sequence comprising SEQ ID NO:19, and     -   (b) sequences which are at least 70%, at least 80%, at least         90%, at least 95%, at least 98%, or at least 99% identical to         the sequences of (a).

The transmembrane linker (ii) and the transporter domain of the EhaA protein (iii) are in particular encoded by a sequence comprising a sequence selected from the group consisting of:

-   -   (a) a nucleotide sequence comprising SEQ ID NO:1,     -   (b) a nucleotide sequence encoding SEQ ID NO:2,     -   (c) nucleotide sequences comprising a sequence being at least         70%, at least 80%, at least 90%, at least 95%, at least 98%, or         at least 99% identical to SEQ ID NO:1 or/and a nucleotide         sequence encoding SEQ ID NO:2, and     -   (d) nucleotide sequences which encodes the polypeptides encoded         by (a), (b) or/and (c) within the scope of the degeneracy of the         genetic code.

The transmembrane linker (ii) and the transporter domain of the EhaA protein (iii) in particular comprises a sequence selected from the group consisting of:

-   -   (a) an amino acid sequence comprising SEQ ID NO:2, and     -   (b) sequences which are at least 70%, at least 80%, at least         90%, at least 95%, at least 98%, or at least 99% identical to         the sequences of (a).

A sequence encoding the transmembrane linker or/and the transporter domain can be obtained from E. coli EhaA sequences.

The sequence of the nucleic acid fusion can have a codon usage adapted to the host cell. In particular, the codon usage of the transmembrane linker sequence, the EhaA transporter domain or/and the passenger can be adapted to the host cell. More particular, the codon usage of the transmembrane linker sequence or/and the EhaA transporter domain can be adapted to the host cell. This is can improve expression if the EhaA transporter domain is heterologous to the host cell. Optimisation of codon usage does usually not affect the amino acid sequence. The EhaA nucleic acid sequence being codon-optimized can have an identity of about 80% identity with the natural EhaA sequence (FIG. 25).

Examples of codon-optimized nucleotide sequences are described in SEQ ID NO:1, 16 and 18.

Step (b) of the method of the present invention refers to culturing the host cell under conditions wherein the nucleic acid fusion is expressed and the expression product comprising the recombinant polypeptide is displayed on the surface of the host cell. The person skilled in the art knows suitable culture conditions, in particular for the host cell species as described herein. The method according to the invention allows for an efficient expression of passenger proteins on the surface of host cells, particularly E. coli or other Gram-negative bacterial cells up to 100 000 or more molecules per cell by using a liquid medium of the following composition: 5 g/l to 20 g/l, preferably about 10 g/l trypton, 2 g/l to 10 g/l, preferably about 5 g/l yeast extract, 5 g/l to 20 g/l, in particular about 10 g/l NaCl and the remaining part water. The medium should possibly contain as little as possible divalent cations, thus preferably Aqua bidest or highly purified water, e.g. Millipore water is used. The liquid medium may contain in addition preferably EDTA in a concentration of 2 μM to 20 μM, in particular 10 μM. Moreover, it contains preferably reducing reagents, such as 2-mercaptoethanol or dithiotreitol or dithioerythritol in a preferred concentration of 2 mM to 20 mM. The reducing reagents favour a non-folded structure of the polypeptide during transport. The liquid medium can further contain additional C-sources, preferably glucose, e.g. in an amount of up to 10 g/l, in order to favour secretion i.e. transfer of the passenger to the surrounding medium. For surface display preferably no additional C-source is added. Preferred culture conditions for Gram-negative cells, such as E. coli, Z. mobilis, P. putida and S. enterica, are described in the Examples.

If the host cell is a Gram-negative bacterium, the polypeptide synthesized in the cytoplasma can be translocated from the cytoplasm into the periplasmic space by crossing the inner membrane. This can be effected by the signal peptide. During maturation, the signal peptide can be cleaved off. The polypepide fusion to be displayed on the surface of a host cell can therefore comprise an amino acid sequence encoded by nucleic acid components (ii) to (iv), as described herein.

The components (i) to (iv) are fused in frame. The components (i) to (iv) in the nucleic acid fusion of the present invention are preferably oriented from 5′ to 3′. In the expression product obtained in step (b), the amino acid sequences encoded by nucleic acid sequences (i) to (iv) are preferably arranged N terminal to C terminal.

The nucleic acid fusion can further comprise at least one nucleic acid sequence encoding an affinity tag. The nucleic acid sequence encoding the affinity tag can flank the portion (ii) encoding the recombinant polypeptide to be displayed. In this embodiment, the nucleic acid sequence encoding the affinity tag can be separated from portion (ii) by a sequence encoding at least one protease recognition sequence. The at least one protease recognition sequence can be a first protease recognition sequence. The at least one protease recognition sequence can be any protease recognition sequence as described herein. Preferably, the at least one protease recognition sequence is independently selected from factor Xa cleavage site, OmpT cleavage site, and TEV protease cleavage site.

The affinity tag can independently be selected from His₆ and epitopes. In particular, the epitope is recognised by a specific antibody, for example a monoclonal antibody. An example of a suitable epitope is the amino acid sequence PEYFK which is recognized by antibody Dü 142 (Spohn et al, 1992).

Furthermore, the nucleic acid fusion can comprise a nucleotide sequence encoding at least one protease recognition sequence, said nucleotide sequence being located between portions (ii) and portion (iii). The at least one protease recognition sequence can be a second protease recognition sequence. The at least one protease recognition sequence can be any protease recognition sequence as described herein. Preferably, the at least one protease recognition sequence is independently selected from factor Xa cleavage site, OmpT cleavage site, and TEV protease cleavage site.

The protease recognition sequence, as used herein, may be a recognition site for an intrinsic protease, i.e. a protease naturally occurring in the host cell, or an externally added protease. For example, the externally added protease may be an IgA protease (cf. EP-A-0 254 090), thrombin or factor X (factor Xa). The intrinsic protease may be e.g. selected from OmpT, OmpK or protease X. The protease may also be TEV.

In the method of the present invention, the portion (ii) encoding the recombinant polypeptide to be displayed can be flanked by at least one sequence comprising a multiple cloning site. The multiple cloning site is suitable for introduction of any further nucleic acid sequence, or for replacement of the portion (ii) encoding the passenger by a nucleotide sequence encoding another passenger.

Preferably, the portion (ii) is flanked by two sequences comprising a multiple cloning site. The first of these sequences is located 5′ of portion (ii). The second of these sequences is located 3′ of portion (ii).

The method of the present invention may comprise preparing a membrane preparation from the cell obtained in step (b). The membrane preparation may comprise membrane particles. The membrane particles may be membrane vesicles. Preferred membrane particles are outer membrane particles. In particular the method of the present invention may comprise preparing outer membrane particles of cells displaying a recombinant polypeptide on the surface, e.g. of Gram-negative bacterial cells. The person skilled in the art knows suitable conditions (e.g. Hantke, 1981, Schultheiss et al., 2002). Typical conditions for preparing membrane particles are employed in the examples of the present invention. Outer membrane particles from a host cell as described herein may be performed by a method comprising the steps:

-   -   (a) treating the host cell with a hydrolase (such as lysozyme)         and optionally with a DNAse. This enzymatic treatment may be         performed at room temperature. The hydrolase hydrolyses the cell         wall within the periplasmic space. The cell wall comprises         peptidoglycans to be hydrolyzed.     -   (b) optionally solubilizing the preparation of (a) with a         tenside, such as Triton X-100, or/and with sarcosine, followed         by optional centrifugation of cell debris. The thus obtained         preparation of outer membrane particles may be centrifuged,         washed and resuspended.

The diameter of the membrane particles may be in the range of 1 nm to 1000 nm, in the range of 50 nm to 500 nm, in the range of 75 to 200 nm, or in the range of 90 to 120 nm. At least 80%, at least 90%, at least 95%, or at least 98% of the membrane particles may have a diameter in a range selected from the ranges described herein.

In a host cell being a Gram-negative bacterium, after translocation, the recombinant passenger remains attached to the surface of the outer membrane by the β-barrel, which is serving as an anchor within the outer membrane. Due to the controlled integration of the β-barrel within the outer membrane, the C terminal part of the β-barrel is directed to the inner side of the outer membrane, whereas the N-terminal part of the linker, to which the recombinant passenger protein is covalently bound, is directed to the outer surface of the outer membrane, i.e. the environment. The recombinant passenger protein has an oriented location after transport, namely it is directed to the cellular surface. The recombinant passenger protein has the identical orientation as the lipopolysaccharide (LPS) layer which may be present in the outer membrane.

Membrane particles of the present invention prepared from the host cell of the present invention comprise the recombinant peptide at the surface directed to the environment. In contrast to the inner membrane which is a unit membrane, the outer membrane of Gram-negative bacteria, in particular E. coli, is asymmetric. The outer membrane may comprise an inner layer comprising phospholipids and an outer layer comprising LPS. LPS is hydrophilic and may contain several negative charges. By using outer membrane particles with anchored passenger proteins by a β-barrel for the coating of carriers, the outer side of the outer membrane, in particular the LPS side will be directed to the surface distal to the carrier. As a consequence the recombinant protein or a domain thereof, which are integrated in the outer membrane by autodisplay, will be directed to the surface distal to the carrier as well. The core part of the membrane particles may stabilize the interaction of the outer membrane layer obtained by applying outer membrane particles to the carrier by hydrophobic interactions and may contain lipoproteins or peptidoglycans.

The skilled person knows suitable methods to determine the degree of identity of nucleic acid sequences and amino acid sequences. Known algorithms, such as BLAST (for nucleic acids) or PBLAST (for amino acid sequences) may be used. A nucleic acid or polypeptide comprising sequences having at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% identity to a given sequence includes fragments of the given nucleic acid or polypeptide.

The polypeptide of the present invention to be displayed on the surface of the cell may be a multimeric polypeptide. The multimeric recombinant polypeptide may be a homodimer, i.e. a polypeptide consisting of two identical subunits or a homomultimer, i.e. a polypeptide consisting of three or more identical subunits. On the other hand, the multimeric recombinant polypeptide may be a heterodimer, i.e. a polypeptide consisting of two different subunits or a heteromultimer consisting of three or more subunits wherein at least two of these subunits are different. For example, the multimeric polypeptide is comprised of a plurality of subunits which form a “single” multimeric polypeptide or a complex of a plurality of functionally associated polypeptides which may in turn be monomeric and/or multimeric polypeptides. It should be noted that at least one subunit of the multimeric recombinant protein may contain at least one prosthetic group as described herein. Further, is should be noted that the nucleic acid fusion may encode a plurality of polypeptide subunits as a polypeptide fusion which when presented on the cell surface forms a functional multimeric polypeptide.

Homodimers or homomultimers may be formed by a spontaneous association of several identical polypeptide subunits displayed on the host cell membrane. Heterodimers or heteromultimers may be formed by a spontaneous association of several different polypeptide subunits displayed on the host cell membrane.

On the other hand, a multimeric recombinant polypeptide may be formed by an association of at least one polypeptide subunit displayed on the host cell membrane, as described herein, and at least one soluble polypeptide subunit added to the host cell membrane. The added subunit may be identical to the displayed subunit or be different therefrom.

Another aspect of the present invention is a recombinant vector comprising the nucleic acid fusion as described herein, operatively linked to an expression control sequence. In particular, the recombinant vector comprises:

-   -   (i) a portion encoding a signal peptide,     -   (ii) a portion encoding a multiple cloning site,     -   (iii) a portion encoding a transmembrane linker, and     -   (iv) a portion encoding the transporter domain of an EhaA         protein.

In this embodiment, the signal peptide is a signal peptide as described herein.

The multiple cloning site is a multiple cloning site as described herein. The multiple cloning site is in particular suitable for integration of a nucleic acid sequence encoding a recombinant polypeptide in frame with portions (i), (ii) and (iv). The transmembrane linker is a transmembrane linker as described herein. The transporter domain of the EhaA protein is a transporter domain of the EhaA protein as described herein. In this embodiment, the nucleic acid sequences (i) to (iv) are in particular arranged from 5′ to 3′.

Another aspect of the present invention is a recombinant host cell comprising the recombinant vector as described herein.

Yet another aspect of the present invention is a method of displaying a recombinant polypeptide on the surface, said method comprising the steps

-   -   (a) providing a recombinant vector comprising a multiple cloning         site (ii), as described herein,     -   (b) inserting a sequence encoding the recombinant polypeptide to         be displayed into the multiple cloning site (ii), and     -   (c) performing the method of displaying a recombinant         polypeptide on the surface of a host cell, as described herein.

Yet another aspect of the present invention is a method for producing a host cell capable of displaying a recombinant polypeptide on the surface, said method comprising the steps

-   -   (a) providing a recombinant vector comprising a multiple cloning         site (ii), as described herein,     -   (b) inserting a sequence encoding the recombinant polypeptide to         be displayed into the multiple cloning site (ii), and     -   (c) performing the method of displaying a recombinant         polypeptide on the surface of a host cell, as described herein.

Yet another aspect of the present invention is a host cell capable of displaying the recombinant polypeptide on the surface. The host cell may be any host cell as described herein. The polypeptide fusion displayed on the cell surface comprises:

-   -   (I) a portion comprising the recombinant polypeptide to be         displayed,     -   (II) a portion comprising a transmembrane linker, and     -   (III) a portion comprising the transporter domain of an EhaA         protein.

The portion (I) is also termed “passenger”, as described herein.

The displayed polypeptide is in particular a functional polypeptide, as described herein.

The portions (I) to (III) of the recombinant polypeptide (polypeptide fusion) displayed by the host cell of the present invention are encoded in particular by the components (ii), (iii), and (iv) of the nucleic acid fusion, as described herein.

Yet another aspect of the present invention is a membrane preparation comprising a recombinant polypeptide. The membrane preparation of the present invention may comprise membrane particles, as described herein.

The membrane preparation may be obtained from a host cell as described herein. The recombinant polypeptide of the may be any recombinant polypeptide as described herein.

Yet another aspect of the present invention is the use of a membrane preparation comprising a recombinant polypeptide in the manufacture of a carrier comprising a recombinant polypeptide.

The membrane preparation of the present invention may be employed for coating a carrier. The carrier may comprise a membrane preparation of the present invention, as described herein.

The carrier may comprise a hydrophobic surface. The hydrophobic surface may have a contact angle of more than 90°. A increasing surface angle of more than 30° indicates a gradually increasing hydrophobicity of a surface. In the present context, a hydrophobic surface may have a contact angle of at least 40°. The surface preferably has a hydrophobicity described by a contact angle of at least 40°, at least 50°, at least 60°, at least 65°, at least 70°.

Contact angles are preferably determined by the sessile drop method. The sessile drop method is a standard method for determining contact angles. Measurements may be performed with a contact angle goniometer. Preferred contact angles of the hydrophobic surface are in a range of 40° to 100°, 50° to 90°, or 60° to 80°.

The surface of the carrier may be a metal surface. A suitable metal surface has a contact angle e.g. in the range of 50° to 80°. A suitable metal may be selected from gold, silver, titanium, aluminium and alloys such as brass. A preferred surface is a gold surface. The gold surface may be employed as it is. An untreated gold surface has a hydrophobicity suitable for the carrier as described herein. A treatment of the gold surface with thiolated hydrocarbons or hydrocarbons with functional groups such as carboxylic acids or hydroxyl groups is not required.

Another preferred surface of the carrier comprises a polymer, for instance a surface usually employed in disposable materials for use in biochemical or/and medical science. The polymer may be an artificial polymer. Examples of artificial polymers include a polymer selected from polystyrenes, polypropylenes, and polycarbonates. The polystyrene may be produced from [2,2]paracyclophane monomers. Polystyrene surfaces may be treated with oxygene plasma introducing OH or/and methylene groups in order to modify the hydrophobicity. Examples of such modified surfaces include Maxi-sorp, Medi-sorp, Multi-sorp, and Poly-sorp surfaces. Another suitable polystyrene surface is Parylene N produced from [2,2]paracyclophane monomers. Yet another suitable surface is Parylene A [Poly(monoamino-p-xylene)]. Especially suitable are surfaces comprising a polymer having a hydrophobicity described by a contact angle of at least 50°. Suitable surfaces are selected from polystyrene, Parylene A, Parylene N, Maxi-sorp, Medi-sorp, Multi-sorp, and Poly-sorp. Preferred surfaces are selected from polystyrene, Parylene A, Parylene N, Maxi-sorp, Medi-sorp, and Poly-sorp.

The surface may comprise a natural polymer. Suitable natural polymers include polybutyrate and cellulose and derivatives thereof. A further surface is provided by latex particles, in particular latex beads.

Yet another surface is provided by C18-modified particles, in particular C18-modified monolithic silica particles. C18 refers to an alkyl group comprising 18 carbon atoms. C18-modified particles are known in the art.

Yet another suitable surface is a glass surface.

The surface may be modified is order to adjust the hydrophobicity. Modification may be performed by chemical treatment (i.e. by oxygen plasma), physical treatment (e.g. by laser irradiation or/and radioactive irradiation), or by mechanical treatment.

The method according to the invention and the host cells according to the invention can be used for a variety of different applications, e.g. as whole cell biofactories or membrane preparation biofactories for chemical synthesis procedures, e.g. for the synthesis of organic substances selected from enzyme substrates, drugs, hormones, starting materials and intermediates for syntheses procedures and chiral substances (cf. Roberts, Chemistry and Biology 6 (1999), R269-R272).

In particular, the method according to the invention and the host cells according to the invention, as described herein, can be used in the chemical synthesis, for example in enzymatically catalyzed enantioselective or/and regioselective steps.

Yet another aspect of the present invention is the surface display of cellulose degrading enzymes (cellulases) on an ethanologenic bacterium, such as a cell selected from Z. mobilis, Z. palmae, P. putida, and Klebsiella spp. by the method of the present invention. An ethanologenic bacterium, such as a cell selected from Z. mobilis, Z. palmae, P. putida, and Klebsiella spp. can provide a whole-cell catalytic system which is able to breakdown cellulose and ferment the formed monomeric sugars to ethanol in a single step.

Klebsiella spp. is in particular Klebsiella oxytoca.

An ethanologenic bacterium, as used herein, is a bactium capable of producing ethanol. Examples of ethanologenic bacteria include, but are not limited to Z. mobilis Z. palmae, P. putida, and Klebsiella spp, in particular Klebsiella oxytoca.

Surface display of a cellulose degrading enzyme (cellulase) on a Z. mobilis cell is preferred. Surface display of a cellulose degrading enzyme (cellulase) on a Z. palmae cell is also preferred. Surface display of a cellulose degrading enzyme (cellulase) on a P. putida cell is also preferred.

A further aspect of the present invention refers to a method of production of ethanol, said method comprising the steps:

-   -   (a) expressing at least one cellulose degrading enzyme on the         surface of an ethanologenic bacterium, such as a cell selected         from Z. mobilis, Z. palmae, P. putida and Klebsiella spp., by         the method of displaying a recombinant polypeptide on the cell         surface, as described herein,     -   (b) contacting the cell of (a) with a cellulose containing         substrate under suitable conditions wherein the at least one         cellulose degrading enzyme expressed on the surface of an         ethanologenic bacterium, such as a cell selected from Z.         mobilis, Z. palmae, P. putida and Klebsiella spp., is capable of         degrading cellulose into ethanol, and     -   (c) obtaining the ethanol.

The skilled person knows suitable condition of (b) and (c).

In the method of production of ethanol, a preferred cell is a Z. mobilis cell. In the method of production of ethanol, another preferred cell is a Z. palmae cell. In the method of production of ethanol, yet another preferred cell is a P. putida cell. In the method of production of ethanol, yet another preferred cell is a Klebsiella spp. cell.

Yet another aspect of the present invention refers to a method of degrading cellulose or/and lignocellulose, said method comprising the steps:

-   -   (a) expressing at least one of an endoglucanase (EC 3.2.1.4), an         exoglucanase (EC 3.2.1.91), an a 3-glucosidase (EC 3.2.1.21), by         the method of the present invention of displaying a recombinant         polypeptide on the surface of a cell,     -   (b) contacting the cell of (a) with a cellulose or/and         lignocellulose containing substrate under suitable conditions         where the at least one enzyme expressed on the surface of the         cell is capable of degrading the cellulose or/and         lignocellulose, and     -   (c) optionally obtaining at least one degradation product of the         cellulose or/and lignocellulose.

In degradation of cellulose or/and lignocellulose, an endocellulase can cleave the beta-1,4-glycosidic bond, in particular in amorphous regions of the cellulose or/and lignocellulose, thereby generating cellulose polysaccharide chains of variable lengths. The cellulose polysaccharide chains include free terminal residues. An exoglucanase can cleave off cellobiose units from the terminal residues of the cellulose polysaccharides. Cellobiose can be converted into glucose by a β-glucosidase.

By the method method of degrading cellulose or/and lignocellulose, the cellulose or/and lignocellulose can be degraded into reducing sugers, in particular into glucose, cellubiose or/and cellulose-polysaccharide chains of variable lengths). It is preferred that the cellulose or/and lignocellulose is degraded into glucose.

The cell can be any cell as described herein. The cell can be selected from Z. mobilis, Z. palmae and Pseudomonas putida. In particular, the cell is a Pseudomonas spp. cell, more particular a Pseudomonas putida cell, most particular Pseudomonas putida KT2440.

In step (a), it is preferred to express a combination of an endoglucanase (EC 3.2.1.4), an exoglucanase (EC 3.2.1.91), or/and a β-glucosidase (EC 3.2.1.21). More preferred is a combination of an endoglucanase (EC 3.2.1.4), an exoglucanase (EC 3.2.1.91), and a β-glucosidase (EC 3.2.1.21).

The at least one of the enzymes of step (a) a can be expressed on a common cell, or each enzyme to be expressed can be expressed on a separate cell.

A preferred exoglucanase of the present invention can be obtained from Clostridium thermocellum.

A preferred exoglucanase of the present invention comprises amino acid positions 38-831 of SEQ ID NO:21, or a sequence being at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% identical to positions 38-831 of SEQ ID NO:21.

A preferred exoglucanase of the present invention is encoded by SEQ ID NO:20, in particular by positions 112-2493 of SEQ ID NO:20, or by a sequence being at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% identical to positions 112-2493 of SEQ ID NO:20.

A preferred endoglucanase of the present invention can be obtained from Bacillus subtilis.

A preferred endoglucanase of the present invention comprises amino acid positions 38-511 of SEQ ID NO:23, or a sequence being at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% identical to positions 38-511 of SEQ ID NO:23.

A preferred endoglucanase of the present invention is encoded by SEQ ID NO:22, in particular by positions 112-1533 of SEQ ID NO:22, or by a sequence being at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% identical to positions 112-1533 of SEQ ID NO:22.

A preferred β-glucosidase of the present invention can be obtained from Clostridium thermocellum.

A preferred β-glucosidase of the present invention comprises amino acid positions 38-488 of SEQ ID NO:25, or a sequence being at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% identical to positions 38-488 of SEQ ID NO:25.

A preferred β-glucosidase of the present invention is encoded by SEQ ID NO:24, in particular by positions 112-1464 of SEQ ID NO:24, or by a sequence being at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% identical to positions 112-1464 of SEQ ID NO:24.

The skilled person knows suitable condition of (b) and (c). Step (b) can be performed at a pH in the range of about 3 to about 7, about 4 to about 6.5, about 5 to about 6.5, or about 5.5 to about 6.5, or at a pH of about 6. A preferred pH is about 6.

Step (b) can be performed at a temperature in the range of about 45° C. to about 65° C., or about 50° C. to about 60° C., or at a temperature of about 55° C. A temperature of about 55° C. is preferred.

In step (b), a buffer can be used in a concentration of about 0.5 M to about 1.5 M, about 0.75 M to about 1.25 M, about 0.9 M to about 1.1 M, or about 1 M. A preferred buffer concentration is 1 M. Any suitable buffer can be used, for example citrate buffer (such as sodium citrate buffer).

Preferred conditions for step (b) include 1 M citrate buffer at a pH of about 6, and 55° C., or 1 M sodium citrate buffer at a pH of about 6, and 55° C.

SEQ ID NOs: 3, 5, 7, 9, 11, 13, 20, 22 and 24 describe specific examples of nucleic acid fusions of the present invention. SEQ ID NOs: 4, 6, 8, 10, 12, 14, 21, 23 and 25 describe polypeptides encoded by SEQ ID NOs: 3, 5, 7, 9, 11 13, 20, 22 and 24, respectively.

SEQ ID NO:3:

Nucleotide Sequence pMATE-MT004, for the Surface Display of 6×His using the MATE System

Annotation of SEQ ID NO:3

feature position pUC ori 2-805 bp T5 promoter 1455-1502 bp CtxB SP sequence 1583-1663 bp 6xHis 1664-1681 bp fXa cleavage site 1682-1693 bp MCS 1694-1717 bp OmpT cleavage site 1718-1729 bp fXa cleavage site (2.nd) 1730-1741 bp PEYFK epitope 1745-1759 bp MATE linker and β-barrel 1760-3226 bp KanR (kanamycine resistance cassette) 3606-4415 bp LacI repressor gene 4431-5262 bp

SEQ ID NO:4

Polypeptide Sequence of Autotransporter Fusion Protein Encoded by pMATE-MT004, for the Surface Display of 6×His using the MATE System

Annotation of SEQ ID NO:4

feature position CtxB SP 1-27 aa 6xHis 28-33 aa fXa cleavage site 34-37 aa MCS 38-45 aa OmpT cleavage site 46-49 aa fXa cleavage site (2.nd) 50-53 aa PEYFK epitope 55-59 aa MATE linker and β-barrel 60-547 aa

SEQ ID NO:5:

Nucleotide Sequence of pMATE-MT006, for the Surface Display of GFP using the MATE System

Annotation of SEQ ID NO:5

feature position pUC ori 2-805 bp T5 promoter 1455-1502 bp CtxB SP sequence 1583-1663 bp 6xHis 1664-1681 bp fXa cleavage site 1682-1693 bp GFPmut2 1694-2410 bp OmpT cleavage site 2411-2422 bp fXa cleavage site (2.nd) 2423-2434 bp PEYFK epitope 2438-2452 bp MATE linker and β-barrel 2453-3919 bp KanR (kanamycine resistance cassette) 4299-5108 bp LacI repressor gene 5191-6252 bp

SEQ ID NO:6 Polypeptide Sequence of the Autotransporter Fusion Protein Encoded by pMATE-MT006, for the Surface Display of GFP using the MATE System

Annotation of SEQ ID NO:6

feature position CtxB SP 1-27 aa 6xHis 28-33 aa fXa cleavage site 34-37 aa GFPmut2 38-276 aa OmpT cleavage site 277-280 aa fXa cleavage site (2.nd) 281-284 aa PEYFK epitope 286-290 aa MATE linker and β-barrel 291-778 aa

SEQ ID NO:7

Nucleotide Sequence of pMATE-SI005, for the Surface Display of 6×His using the MATE System in a Broad Range of Bacterial Hosts

Annotation of SEQ ID NO:7

feature position AraC 1-895 bp araBAD promoter 916-1188 bp CtxB SP sequence 1231-1311 bp 6xHis 1312-1329 bp fXa cleavage site 1330-1341 bp MCS 1342-1365 bp OmpT cleavage site 1366-1377 bp fXa cleavage site (2.nd) 1378-1389 bp PEYFK epitope 1393-1407 bp MATE linker and β-barrel 1408-2874 bp pBBR1 rep gene (broad host rep) 3196-3858 bp Kanamycine resistance cassette 5392-6186 bp

SEQ ID NO:8

Polypeptide Sequence of the Autotransporter Fusion Protein Encoded by pMATE-SI005, for the Surface Display of 6×His using the MATE System in a Broad Range of Bacterial Hosts

Annotation of SEQ ID NO:8

feature position CtxB SP 1-27 aa 6xHis 28-33 aa fXa cleavage site 34-37 aa MCS 38-45 aa OmpT cleavage site 46-49 aa fXa cleavage site (2.nd) 50-53 aa PEYFK epitope 55-59 aa MATE linker and β-barrel 60-547 aa

SEQ ID NO:9

Nucleotide Sequence of pMATE-SI010, for the Surface Display of estA Catalytic Domain using the MATE System in a Broad Range of Bacterial Hosts

Annotation of SEQ ID NO:9

feature position AraC   1-895 bp araBAD promoter  916-1188 bp CtxB SP sequence 1231-1311 bp 6xHis 1312-1329 bp fXa cleavage site 1330-1341 bp estA catalytic domain 1342-2388 bp OmpT cleavage site 2389-2400 bp fXa cleavage site (2.nd) 2401-2412 bp PEYFK epitope 2416-2430 bp MATE linker and β-barrel 2431-3897 bp pBBR1 rep gene (broad host rep) 4219-4881 bp Kanamycine resistance cassette 6415-7209 bp

SEQ ID NO:10

Polypeptide Sequence of the Autotransporter Fusion Protein Encoded by pMATE-SI010, for the Surface Display of B. gladioli EstA Esterase Domain using the MATE System in a Broad Range of Bacterial Hosts

Annotation of SEQ ID NO:10

feature position CtxB SP   1-27 aa 6xHis  28-33 aa fXa cleavage site  34-37 aa EstA catalytic domain  38-386 aa OmpT cleavage site 387-390 aa fXa cleavage site (2.nd) 391-394 aa PEYFK epitope 396-400 aa MATE linker and β-barrel 401-888 aa

SEQ ID NO:11

Nucleotide Sequence of pMATE-SI012, for the Surface Display of estA Catalytic Domain using the MATE System in a Broad Range of Bacterial Hosts

Annotation of SEQ ID NO:11

feature position Kanamycine resistance cassette  541-1335 bp Mob gene 1593-2592 bp pBBR1 rep gene (broad host rep) 3585-4247 bp AraC 4310-5204 bp araBAD promoter 5225-5497 bp CtxB SP sequence 5540-5620 bp 6xHis 5621-5638 bp fXa cleavage site 5639-5650 bp estA catalytic domain 5651-6697 bp OmpT cleavage site 6698-6709 bp fXa cleavage site (2.nd) 6710-6721 bp PEYFK epitope 6725-6739 bp MATE linker and β-barrel 6740-8206 bp

SEQ ID NO:12

Polypeptide Sequence of the Autotransporter Fusion Protein Encoded by pMATE-SI012, for the Surface Display of B. gladioli EstA Esterase Domain using the MATE System in a Broad Range of Bacterial Hosts

Annotation of SEQ ID NO:12

feature position CtxB SP   1-27 aa 6xHis  28-33 aa fXa cleavage site  34-37 aa EstA catalytic domain  38-386 aa OmpT cleavage site 387-390 aa fXa cleavage site (2.nd) 391-394 aa PEYFK epitope 396-400 aa MATE linker and β-barrel 401-888 aa

SEQ ID NO:13

Nucleotide Sequence of pMATE-SI015, for the Surface Display of 6×His-mCherry using the MATE System

Annotation of SEQ ID NO:13

feature position pUC ori   2-805 bp T5 promoter 1455-1502 bp CtxB SP sequence 1583-1663 bp 6xHis 1664-1681 bp mCherry 1682-2389 bp OmpT cleavage site 2390-2401 bp fXa cleavage site 2402-2413 bp PEYFK epitope 2417-2431 bp MATE linker and β-barrel 2432-3898 bp KanR (kanamycine resistance cassette) 4278-5087 bp Lacl repressor gene 5170-6252 bp

SEQ ID NO:14

Polypeptide Sequence of the Autotransporter Fusion Protein Encoded by pMATE-51015, for the Surface Display of 6×His-mCherry using the MATE System

Annotation of SEQ ID NO:14

feature position CtxB SP   1-27 aa 6xHis  28-33 aa mCherry  34-269 aa OmpT cleavage site 270-273 aa fXa cleavage site (2.nd) 274-277 aa PEYFK epitope 279-283 aa MATE linker and β-barrel 284-771 aa

SEQ ID NO:20

Nucleotide Sequence of the Autotransporter Fusion Gene Encoded by pMATE-exoglucanase, for the Surface Display of an Exoglucanase using the pMATE System (length 4002 bp)

Annotation of SEQ ID NO:20

Feature position CtxB SP sequence    1-81 bp 6xHis   82-99 bp fXa cleavage site  100-111 bp exoglucanase  112-2493 bp OmpT cleavage site 2494-2505 bp fXa cleavage site (2.nd) 2506-2517 bp PEYFK epitope 2521-2535 bp MATE linker and β-barrel 2536-4002 bp

SEQ ID NO:21

Polypeptide Sequence of the Autotransporter Fusion Protein Encoded by pMATE-exoglucanase, for the Surface Display of an Exoglucanase using the pMATE System (length 1333 aa)

Annotation of SEQ ID NO:21

Feature position CtxB SP   1-27 aa 6xHis  28-33 aa fXa cleavage site  34-37 aa exoglucanase  38-831 aa OmpT cleavage site 832-835 aa fXa cleavage site (2.nd) 836-839 aa PEYFK epitope 841-845 aa MATE linker and β-barrel 846-1333 aa 

SEQ ID NO:22

Nucleotide Sequence of the Autotransporter Fusion Gene Encoded by pMATE-endoglucanase, for the Surface Display of an Endoglucanase using the pMATE System (length 3042 bp)

Annotation of SEQ ID NO:22

Feature position CtxB SP sequence    1-81 bp 6xHis   82-99 bp fXa cleavage site  100-111 bp endoglucanase  112-1533 bp OmpT cleavage site 1534-1545 bp fXa cleavage site (2.nd) 1546-1557 bp PEYFK epitope 1561-1575 bp MATE linker and β-barrel 1576-3042 bp

SEQ ID NO:23

Polypeptide Sequence of the Autotransporter Fusion Protein Encoded by pMATE-endoglucanase, for the Surface Display of an Endoglucanase using the pMATE System (length 1013 aa)

Annotation of SEQ ID NO:23

Feature position CtxB SP   1-27 aa 6xHis  28-33 aa fXa cleavage site  34-37 aa endoglucanase  38-511 aa OmpT cleavage site 512-515 aa fXa cleavage site (2.nd) 516-519 aa PEYFK epitope 521-525 aa MATE linker and β-barrel 562-1013 aa 

SEQ ID NO:24

Nucleotide Sequence of the Autotransporter Fusion Gene Encoded by pMATE-f3-glucosidase, for the Surface Display of a β-glucosidase using the pMATE System (length 2973 bp)

Annotation of SEQ ID NO:24

Feature position CtxB SP sequence    1-81 bp 6xHis   82-99 bp fXa cleavage site  100-111 bp β-glucosidase  112-1464 bp OmpT cleavage site 1465-1476 bp fXa cleavage site (2.nd) 1477-1488 bp PEYFK epitope 1492-1506 bp MATE linker and β-barrel 1507-2973 bp

SEQ ID NO:25

Polypeptide Sequence of the Autotransporter Fusion Protein Encoded by pMATE-f3-glucosidase, for the Surface Display of a β-glucosidase using the pMATE System (length 990 aa).

Annotation of SEQ ID NO:25

Feature position CtxB SP   1-27 aa 6xHis  28-33 aa fXa cleavage site  34-37 aa β-glucosidase  38-488 aa OmpT cleavage site 489-492 aa fXa cleavage site (2.nd) 493-496 aa PEYFK epitope 498-502 aa MATE linker and β-barrel 503-990 aa

Further, the present invention shall be further illustrated by the following figures and examples:

FIG. 1: Use of the MATE system for the transport of 6×His to the outer membrane. E. coli BL21 cells were grown in LB medium to OD₆₀₀ of 0.5. Protein expression was induced by the addition of 1 mM IPTG, and the cells were harvested after 1 hour. Outer membrane proteins were isolated according to the modified method of Hantke et al. (1981). (A) SDS-PAGE of outer membrane proteins. (B) Western blot with antibody against 6×His. M=PageRuler prestained protein marker, 1=cells with negative control plasmid, expressing an unrelated peptide with AIDA-I (pST005), 2=cells with empty vector (pJExpress401), 3=cells with pMATE-MT004 without the addition of IPTG, 4=cells with pMATE-MT004 with 1 mM IPTG for the induction of protein expression. The arrow indicates the band associated with the MATE fusion protein. Equal amounts of protein were loaded in the gels used for Coomassie staining and Western blots.

FIG. 2: Protease accessibility assay to confirm the surface display of GFP in E. coli with the MATE system. E. coli UT5600 cells containing pMATE-MT006 were harvested after the induction of protein expression with IPTG, and outer membrane proteins were isolated according to the modified method of Hantke (1981). (A) SDS-PAGE with Coomassie stain. (B) Western blot with polyclonal rabbit anti-GFP. M=PageRuler prestained protein marker, 1=cells without induction of protein expression (no IPTG), 2=cells with induction of protein expression, 3=cells with induction of protein expression and trypsin treatment. Samples were diluted 1:20 in loading buffer for Western blotting.

FIG. 3: Secretion of GFP into the cell growth media using the MATE system, based on the cleavage by the E. coli protease OmpT. E. coli cells containing pMATE-MT006 were grown in LB medium until OD₆₀₀ 0.5, and then expression of the fusion protein was induced with IPTG. After 1.5 h the cells were removed from the growth media by centrifugation followed by filtration. The proteins in the media were then concentrated by TCA precipitation and analysed with 12.5% SDS-PAGE. (A) SDS-PAGE with Coomassie stain showing all proteins released into the growth media. (B) Western blot to detect GFP released into the growth media c). M=PageRuler prestained protein marker, 1=E. coli UT5600 (OmpT negative strain), 2=E. coli UT2300 (OmpT positive parent strain). Equal amounts of protein were loaded for SDS-PAGE and Western blot.

FIG. 4: Sample of mCherry and its negative control harvested from the supernatant of OmpT-positive and OmpT-negative E. coli strains. The RFP mCherry was secreted into the cell growth media using the pMATE-System, based on the cleavage by the E. coli protease OmpT. The OmpT-positive E. coli strain UT3200 and its derivate OmpT-negative strain E. coli UT5600 harbouring pMATE-SI015 were grown in LB medium until OD₆₀₀ 0.5, and then expression of the fusion protein was induced with 1 mM IPTG for 24 h. Cells were removed from the growth media by centrifugation. The supernatant of both strains was loaded on a Ni-NTA column for purification of 6×His mCherry.

FIG. 5: SDS-PAGE with Coomassie stain of putative secreted affinity purified 6×His mCherry from OmpT-positive E. coli UT2300 and its derivate OmpT-negative strain E. coli UT5600 each harbouring the pMATE-SI015 plasmid. E. coli cells containing pMATE-SI015 were grown in LB media until OD₆₀₀ 0.5, and then expression of the fusion protein was induced with 1 mM IPTG. After 24 h the cells were removed from the growth media by centrifugation. The supernatant of both strains was loaded on a Ni-NTA column. 6×His proteins were eluted with 500 mM imidazole. After protein concentration, the eluate was loaded on a 10% polyacrylamide gel and subsequently stained with Coomassie. M=PageRuler prestained protein marker, 1=E. coli UT2300 harbouring pMATE-SI015 (OmpT positive parent strain), 2=E. coli UT5600 harbouring pMATE-SI015 (OmpT negative strain).

FIG. 6: Western blot showing the release of surface-displayed GFP into the supernatant by Factor Xa protease. Protein expression in E. coli UT5600 cells containing pMATE-MT006 was induced with 1 mM IPTG. Cells were washed and incubated for 16 h in buffer containing Factor Xa. Proteins were concentrated from the supernatant, separated by 12.5% SDS-PAGE. Western blotting was conducted with a polyclonal 1° against GFP as described in the materials and methods. M=PageRuler prestained protein marker, 1=Factor Xa protease alone 2=supernatant from cells without induction of protein expression (no IPTG), 3=supernatant from cells without induction of protein expression (no IPTG)+Factor Xa protease, 4=supernatant from cells with induction of protein expression, 5=supernatant from cells with induction of protein expression+Factor Xa protease.

FIG. 7: Structure of the EstA-autotransporter fusion protein. Illustration of the parts from the fusion protein necessary for the surface display of the B. gladioli estA catalytic domain using the MATE system. SP=signal peptide. Restriction sites used for cloning are underlined.

FIG. 8: Photometric esterase activity assay, based on the release of p-nitrophenol from pnitrophenyl acetate by E. coli whole cells. Cells contained either a MATE expression plasmid (pMATE-SI010) or an Autodisplay expression plasmid (pES01) for the surface expression of the estA esterase as an autotransporter passenger.OD₆₀₀ of cells in the assay was adjusted to 0.2.

FIG. 9: Photometric activity assay after protease digestion. Release of pnitrophenol from pnitrophenyl acetate by either proteinase K treated or untreated whole cells of S. enterica and S. enterica pMATE-SI010 after 2 h of induction with 0.5% L-arabinose at 30° C. Final OD₆₀₀ in assay was adjusted to 0.2.

FIG. 10: Photometric esterase activity assay of whole cells, based on the hydrolysis of pnitrophenyl acetate. Protein expression was induced for 2 h with 0.5% L-arabinose at 30° C. Final OD₆₀₀ in assay was adjusted to 0.2.

FIG. 11: Photometric esterase activity assay of whole cells based on the hydrolysis of pnitrophenyl acetate. Protein expression was induced by 2 h with 0.5% L-arabinose at 30° C. (A) E. coli UT5600 or (B) P. putida KT2440.

FIG. 12: Flow cytometry analysis of cells displaying the EstA catalytic domain on the surface. After cleavage of the signal peptide, the Nterminal region of the EstA fusion protein is expected to contain a 6×His affinity tag. Cells of P. putida KT2440 were analysed after incubation of the samples with two antibodies, a primary monoclonal anti-6×His antibody and a secondary fluorescein-labelled detection antibody. (A) P. putida KT2440 cells without plasmid (negative control) and (B) P. putida KT2440 pMATE-SI010 cells displaying EstA catalytic domain on the surface.

FIG. 13: Protein content and esterase activity of whole cells expressing EstA using the MATE system in E. coli and P. putida. Cells were grown until OD₆₀₀ 0.6, and gene expression induced by 0.5% (w/v) L-arabinose for 2 h at 30° C. (200 rpm). Cell suspensions were adjusted to an OD of 1 and after harvest. Proteins from whole cells were boiled in SDS-PAGE sample buffer for 30 minutes. The amount of protein loaded on each lane was normalised based on the optical density of the cell solution. (A) SDS-PAGE followed by Coomassie stain for total protein (B) SDS-PAGE followed by renaturation of the enzymes, and ingel stain for esterase activity (B) Designations: M: PageRuler prestained protein marker, 1: control, E. coli UT5600 without plasmid, 2: E. coli UT5600 pMATE-SI010 (EstA as passenger), 3: P. putida KT2440 pMATE-SI010 (EstA as passenger), 4: control, P. putida KT2440 pMATE-SI005 (6×His as passenger).

FIG. 14: Protein content and esterase activity of outer membranes after the expression of EstA using the MATE system in E. coli and P. putida. SDS-PAGE (10% w/v) analysis of outer membrane preparations and outer membrane preparations from whole cells after treatment with proteinase KK. Outer membrane proteins were isolated from E. coli UT5600 and P. putida

KT2440 strains. Cells were grown until OD₆₀₀ 0.6, and protein expression induced by 0.5% (w/v) Larabinose for 2 h at 30° C. (200 rpm). Cells were either used directly for preparations of outer membranes or with a prior incubation step with proteinase K and subsequent outer membrane preparation. (A)

Coomassie stain and (B) esterase activity stain after renaturation of enzymes. M: PageRuler prestained protein marker, 1: control, E. coli UT5600 without plasmid, 2: control, proteinase K treated E. coli UT5600 without plasmid, 3: E. coli UT5600 pMATE-SI010 (EstA as passenger), 4: proteinase K treated E. coli UT5600 pMATE-SI010, 5: P. putida KT2440 pMATE-SI010, 6: proteinase K treated P. putida KT2440, 7: control, P. putida KT2440 pMATE-SI005 (6×His as passenger), 8: control, proteinase KK treated P. putida KT2440 pMATE-SI005.

FIG. 15: Comparison of surface displayed esterase activity in E. coli and P. putida using the MATE system after correcting for intracellular activity. Data was reanalysed from FIG. 11. The activity from strains treated with proteinase K was subtracted from the overall activity seen in the whole cell assay.

FIG. 16: SDS-PAGE (A) and Western Blot (B) analyses of Z. mobilis outer membrane preparations. Z. mobilis cells were cultivated in ZM Medium at 30° C., 60 rpm until OD₅₇₅ reached 0.6. Protein expression was, when needed, induced by addition of L-arabinose to a final concentration of 0.2% and incubation for further 2 h. Cells were then harvested and either used directly for preparations of outer membranes or incubated with 2.5% trypsin in 0.2 mol/L Tris-HCl pH 8.0 for 1 h at 37° C., 200 rpm prior to outer membrane protein isolation. Proteins were then separated by SDS-PAGE and (A) Coomassie stained or (B) transferred to a nitrocellulose membrane stained with anti-6×His primary antibodies. M: PageRuler prestained protein marker, 1: Z. mobilis without plasmid, induced, 2: Z. mobilis with plasmid, induced, 3: Z. mobilis with plasmid, not induced, 4: Z. mobilis with plasmid, induced and treated with Trypsin prior to outer membrane protein isolation.

FIG. 17: Esterase acitivity staining of Z. mobilis outer membrane preparations. Z. mobilis cells were cultivated in ZM Medium at 30° C., 60 rpm until OD₅₇₈ reached 0.6. Protein expression was, when needed, induced by addition of L-arabinose to a final concentration of 0.2% and incubation for further 2 h. Cells were then harvested and either used directly for preparations of outer membranes or incubated with 2.5% trypsin in 0.2 mol/L Tris-HCl pH 8.0 for 1 h at 37° C., 200 rpm prior to outer membrane protein isolation. Proteins were then separated by SDS-PAGE and esterase activity stained. M: PageRuler prestained protein marker, 1: Z. mobilis without plasmid, induced, 2: Z. mobilis with plasmid, induced, 3: Z. mobilis with plasmid, not induced, 4: Z. mobilis with plasmid, induced and treated with Trypsin prior to outer membrane protein isolation.

FIG. 18: Plasmid map of pMATE-MT004.

FIG. 19: Plasmid map of pMATE-MT006.

FIG. 20: Plasmid map of pMATE-SI005.

FIG. 21: Plasmid map of pMATE-SI009.

FIG. 22: Plasmid map of pMATE-SI010.

FIG. 23: Plasmid map of pMATE-SI012.

FIG. 24: Plasmid map of pMATE-SI015.

FIG. 25: Alignment of EhaA (natural sequence and codon-optimized sequence) and AIDA-I.

FIG. 26: Plasmid map of pMATE-PT004.

FIG. 27: SDS-PAGE of outer membrane isolates of Z. palmae cells. Cells were cultivated as described previously and, when necessary, induced at OD₅₇₈=0.5 for one hour with 0.2% arabinose. Outer membrane fraction was isolated, separated in a 12.5% polyacrylamide gel and stained with Coomassie Brilliant Blue. 1: Prestained protein ladder, molecular weight in kDa is indicated on the left. 2: Z. palmae wildtype cells, induced. 3: Z. palmae pMATE-PT004 cells, not induced. 4: Z. palmae pMATE-PT004 cells, induced. 5: Z. palmae pMATE-PT004 cells, induced and digested with trypsin.

FIG. 28: Photometric CMC hydrolysis activity of Z. palmae whole cells based on the detection of reduced sugar formation via DNS assay. Cells were cultivated as described above and, when necessary, induced at OD₅₇₈=0.5 for one hour with 0.2% arabinose, adjusted to OD₅₇₈=25 and incubated with 1% CMC in sodium-citrate buffer, pH 6.0, for ten minutes. Cells were then removed and the supernatant applied to a DNS assay. Absorption values of blank samples (buffer and substrate) were substracted from the absorption values of cell samples.

FIG. 29: Photometric CMC hydrolysis activity of Z. mobilis whole cells based on the detection of reduced sugar formation via DNS assay. Cells were cultivated as described above and, when necessary, induced at OD₅₇₈=0.5 for one hour with 0.2% arabinose, adjusted to OD₅₇₈=25 and incubated with 1% CMC in sodium-citrate buffer, pH 6.0, for ten minutes. Cells were then removed and the supernatant applied to a DNS assay. Absorption values of blank samples (buffer and substrate) were substracted from the absorption values of cell samples.

FIG. 30: FACS-histogram of Z. palmae wildtype cells, treated with anti-6×His 1° antibody and Dylight-633 conjugated 2° antibody; event count approx. 50,000.

FIG. 31: FACS-histogram of induced Z. palmae pMATE-PT004 cells, treated with anti-6×His 1° antibody and Dylight-633 conjugated 2° antibody; event count approx. 50,000.

FIG. 32: Use of the pMATE system for the transport of cellulases to the outer membrane of P. putida. Cells were grown at 30° C. in LB medium to OD₅₇₈ of 0.5. Protein expression was induced by the addition of 0.2% (w/v) L-arabinose for 4 h at 30° C. Cells were harvested and the outer membrane proteins were isolated according to the modified method of Hantke et al. (1981). SDS PAGE of outer membrane proteins was stained with Coomassie. M: prestained protein marker, 1: P. putida KT2440 pMATE-exoglucanase without L-arabinose, 2: P. putida KT2440 pMATE-exoglucanase with L-arabinose, 3: P. putida KT2440 pMATE-endoglucanase without L-arabinose, 4: P. putida KT2440 pMATE-endoglucanase with L-arabinose, 5+9: P. putida KT2440 without plasmid, non-induced, 6: P. putida KT2440 without plasmid, induced, 7: P. putida KT2440 pMATE-3-glucosidase without L-arabinose, 8: P. putida

KT2440 pMATE-β-glucosidase with L-arabinose. The arrow indicates the band associated with the pMATE fusion protein.

FIG. 33: Photometric exoglucanase activity assay of whole cells based on the hydrolysis of p-nitrophenyl-β-D-cellobioside. Protein expression was induced for 4 h with 0.2% L-arabinose at 30° C. Final OD₅₇₈ of cells was adjusted to 0.5.

FIG. 34: Photometric endoglucanase activity assay of whole cells based on the detection of reducing sugars released from carboxymethylcellulose via DNS assay. Protein expression was induced for 4 h with 0.2% L-arabinose at 30° C. Final OD₅₇₈ of cells was adjusted to 20.

FIG. 35: Photometric β-glucosidase activity assay of whole cells based on the hydrolysis of p-nitrophenyl-β-D-glucopyranoside. Protein expression was induced for 4 h with 0.2% L-arabinose at 30° C. Final OD₅₇₈ of cells was adjusted to 20.

FIG. 36: Total pMATE-cellulase activity using the FPA at 55° C. Reducing sugars released from filter paper was detected via DNS assay. BS: Blank substrate (filter paper in buffer); pMATE-cellulase-mix: exoglucanase, endoglucanase and β-glucosidase expressing cells were mixed in equal parts. Protein expression was induced for 4 h with 0.2% L-arabinose at 30° C. Final OD₅₇₈ of total cells was adjusted to 50.

FIG. 37: EFB hydrolysis by pMATE-cellulases at 55° C. for 4 d. Reducing sugars released from 2.5% (dry weight) was detected via DNS assay. BS: Blank substrate (EFB in buffer); pMATE-cellulase-mix: exoglucanase, endoglucanase and β-glucosidase expressing cells were mixed in equal parts. Protein expression was induced for 4 h with 0.2% L-arabinose at 30° C. Final OD₅₇₈ of total cells was adjusted to 20.

FIG. 38: Plasmid map of pMATE-exoglucanase. Annotation of the pMATE-exoglucanase plasmid:

Feature position AraC   4-902 bp araBAD promoter  912-1184 bp CtxB SP sequence 1227-1307 bp 6xHis 1308-1325 bp fXa cleavage site 1326-1337 bp exoglucanase 1338-3719 bp OmpT cleavage site 3720-3731 bp fXa cleavage site (2.nd) 3732-3743 bp PEYFK epitope 3747-3761 bp MATE linker and β-barrel 3762-5228 bp pBBR1 rep gene (broad host rep) 5550-6212 bp KanR (kanamycine resistence cassette) 7746-8540 bp

FIG. 39: Plasmid map of pMATE-endoglucanase. Annotation of the pMATE-endoglucanase plasmid:

Feature position AraC   4-902 bp araBAD promoter  912-1184 bp CtxB SP sequence 1227-1307 bp 6xHis 1308-1325 bp fXa cleavage site 1326-1337 bp endoglucanase 1338-2759 bp OmpT cleavage site 2760-2771 bp fXa cleavage site (2.nd) 2772-2783 bp PEYFK epitope 2787-2801 bp MATE linker and β-barrel 2802-4268 bp pBBR1 rep gene (broad host rep) 4590-5252 bp KanR (kanamycine resistence cassette) 6786-7580 bp

FIG. 40: Plasmid map of pMATE-β-glucosidase. Annotation of the pMATE-beta-glucosidase plasmid:

Feature position AraC   4-902 bp araBAD promoter  912-1184 bp CtxB SP sequence 1227-1307 bp 6xHis 1308-1325 bp fXa cleavage site 1326-1337 bp β-glucosidase 1338-2690 bp OmpT cleavage site 2691-2702 bp fXa cleavage site (2.nd) 2703-2714 bp PEYFK epitope 2718-2732 bp MATE linker and β-barrel 2733-4199 bp pBBR1 rep gene (broad host rep) 4521-5183 bp KanR (kanamycine resistence cassette) 6717-7511 bp

EXAMPLE 1

Surface Expression of 6×His in E. coli using the MATE System

In this example, the MATE system is used to display a short peptide (6×His) on the surface of E. coli.

Bacterial Strains

E. coli BL21 (8, F, dcm, ompT, Ion, hsdS(rB⁻ mB⁻), gal) and E. coli BL21 (DE3) (B, F, dcm, ompT, Ion, hsdS(rB⁻ mB⁻), gal □(DE3)) were used for expression experiments.

Plasmid Construction

The gene encoding the pMATE autotransporter fusion protein was synthesised commercially in the pJexpress-401 plasmid vector (DNA2.0, USA) to create pMATEMT004. The pJExpress401 plasmid backbone contained an rrnB1/B2 terminator, kanamycin resistance gene, LacI repressor, pUC on as well as rpn and bla terminators. Expression of the fusion protein was under the transcriptional control of an Isopropyl β-D-1-thiogalactopyranoside (IPTG) inducible T5 promoter.

The fusion protein included an N-terminal signal peptide from the cholera toxin B subunit (CtxB), a 6×His tag, a multiple cloning site, and the autotransporter domain, which consists of a linker and Pbarrel region.

Protease cleavage sites (Factor Xa and OmpT) were incorporated after the multiple cloning site. A second Factor Xa cleavage site was inserted after the 6×His, for removal of this affinity tag after purification. An epitope (PEYFK) for a monoclonal antibody (Dü142) was inserted after the protease cleavage sites.

The pMATE contains the EhaA autotransporter domain (GenBank Accession No. Q8X6C1). This autotransporter domain has never before been used for the display of recombinant proteins in bacteria; however the full native protein is known to increase cell-cell interactions when overexpressed in E. coli (Wells et al. 2008). To define the border between the original passenger and the autotransporter domain, we aligned the EhaA polypeptide sequence against the Cterminal AIDAI fragment used for Autodisplay (GenBank Accession No. Q03155, Maurer et al. 1997). The sequence encoding the signal peptide and EhaA fragment was codon optimised for E. coli according the algorithm of Welch et al. (2009). The codon usage was further altered to remove some restriction sites, and to minimise predicted RNA 2° structure in the region encoding the CtxB signal peptide.

SDS-PAGE and Western Blot 40 ml of LB media was inoculated with 1 ml from an overnight culture of E. coli BL21 or BL21 (DE3) containing the plasmid of interest. The cultures were then incubated at 37° C., 200 rpm until they reached an OD₆₀₀ of 0.5. Protein expression was induced by the addition of 1 mM IPTG, and the cells harvested one hour after induction. Outer membrane proteins were prepared according to the rapid isolation protocol of Hantke (1981) with modifications as described previously (Jose and von Schwichow, 2004). Proteins were separated with 10% sodium-dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) (Laemmli 1970). Proteins were visualised after SDS-PAGE by staining with Coomassie Brilliant Blue R250. For Western blots, proteins were transferred onto a nitrocellulose membrane using standard electroblotting techniques (Mini-trans blot, Bio-Rad, USA). For the 1° antibody we used a mouse monoclonal anti-6-His IgG (Dianova, Germany). For the 2° antibody we used a horseradish peroxidase-conjugated anti-mouse IgG (Antibodies-online, USA). All solutions for Western blotting were based on Tris/Cl buffered saline (TBS, pH 7.4). The membrane was first blocked with 3% BSA, and incubated with a 1:1000 of 1° antibody for 3 hours. The membrane was then washed in TBS, and incubated for 2 hours with a 1:6000 dilution of 2° antibody. The blot was then washed and incubated with Pierce enhanced chemiluminescent (ECL) western blotting substrate (Thermo Scientific, USA), exposed to X-ray film. Results and Discussion

SDS-PAGE and Western blots revealed a protein of 60-65 kDa in the outer membrane of E. coli containing pMATE-MT004 (FIG. 1). The nucleotide sequence of pMATE-MT004 is described by SEQ ID NO:3 (FIG. 18). The predicted size of the autotransporter fusion protein with 6×His as a passenger was 57.6 kDa after cleavage of the N-terminal signal peptide. The autotransporter fusion protein encoded by pMATE-MT004 is described by SEQ ID NO:4. The fusion protein was visible in all cases as a single band after SDS-PAGE, confirming that the MATE system did not suffer from limitations in the sec secretion or signal peptidase cleavage. The fusion protein was also visible from cells without the induction of protein expression (no IPTG). This suggests some “leaky” expression of the T5 promoter under these conditions, although the protein band seen after SDS-PAGE was much more intense after induction with IPTG.

Overall, this example shows that the MATE system can transfer a peptide to the outer membrane of E. coli. It shows that the 6×His is a suitable epitope for Western blotting, in order to detect the location of the fusion protein after SDS-PAGE.

EXAMPLE 2

Surface Expression of GFP in E. coli using the MATE System

In this example, the MATE system is used to display a fulllength protein (GFP) on the surface of E. coli.

Bacterial Strains

E. coli Stellar cells were used for cloning experiments [F−, endA1, supE44, thi-1, recA1, relA1, gyrA96, phoA, ϕ80d lacZΔ M15, Δ (lacZYA—argF) U169, Δ (mrr—hsdRMS—mcrBC), ΔmcrA, λ—] (Clontech, USA). Expression experiments were carried out in Escherichia coli UT5600 [F⁻, ara-14, leuB6, secA6, lacY1, proC14, tsx-67, Δ(ompT-fepC)266, entA403, trpE38, rfbD1, rpsL109(Str^(r)), xyl-5, mtl-1, thi-1] (Grodberg and Dunn, 1988).

Plasmid Construction

The gene encoding GFP (GFPmut2) was inserted into pMT004 to form pMATE-MT006 (FIG. 19) using ligation independent cloning techniques (In-Fusion Eco-Dry kit, Clontech). The nucleotide sequence of pMATE-MT006 is described by SEQ ID NO:5. The autotransporter fusion protein encoded by pMATE-MT004 is described by SEQ ID NO:6. The GFP insert was amplified from the plasmid pKE19 using the primers MT15 (GCTCGTCGTGCTATTGAGGGCCGCATCCCGG) and MT16 (ACGACCTTCGATATGATGGTGATGGTGGTGGGT) and polymerase chain reaction (PCR). The backbone plasmid was amplified with PCR using the primers MT17 (CATATCGAAGGTCGTatgagtaaaggagaagaacttttc) and MT18 (AATAGCACGACGAGCgcctttgtatagttcatccatgcc), which contained a 15 by overlap to the PCR product of GFP, as required for InFusion cloning. The two PCR Products were joined to form the plasmid pMATE-MT006 using standard In-Fusion techniques (Eco-Dry kit, Clontech) and transformed into E. coli Stellar chemically competent cells (Clontech).

Protease Accessibility Assay

E. coli UT5600 cells containing pMATE-MT006 were grown in LB medium to OD₆₀₀ of 0.5. Protein expression was induced by the addition of 1 mM IPTG, and the cells were harvested after 1 hour. For trypsin treatment, cells were incubated with 1.2 μg·ml⁻¹ of Trypsin (6000 NFU.ml¹) while shaking for 1 hour at 37° C. Outer membrane proteins were isolated, and proteins were separated by 12.5% SDS-PAGE. Gels were stained for total protein with Coomassie Brilliant Blue R-250. For Western blots, proteins were transferred to PVDF membranes (Mini-trans blot, Bio-Rad, USA). The blot was blocked in TBS containing 5% milk powder, and incubated with a 1:2000 dilution of polyclonal rabbit anti-GFP IgG (GeneTex #GTX26556) overnight at 4° C. while shaking. The membrane was then washed with 0.1% Tween in TBS, and incubated for 2 hours with a 1:10 000 dilution of 2° antibody, horseradish peroxidase coupled anti-rabbit IgG (Promega #W401B). Horseradish peroxidase activity was detected with luminol reagent (sc-2048, SantaCruz Biotechnology, USA) and a chemiluminescence imager (Chemocam, Intas).

Pierce enhanced chemiluminescent (ECL) western blotting substrate (Thermo Scientific, USA), and viewed with an ECL imager.

Results and Discussion

A protein of approximately 105 kDa was found in the outer membrane in cells expressing GFP in the MATE system in E. coli. The apparent molecular weight is higher than the expected size of the fusion protein after cleavage of the Nterminal signal peptide (84 kDa). The 105 kDa band was detected with the antiGFP antibody (FIG. 2), confirming that it corresponded to the GFPautotransporter fusion protein.

We conducted a protease accessibility test to confirm the passenger domain was exposed to the surface. The protease trypsin is too large to enter the cell, therefore the trypsin accessibility of an Nterminal passenger domain demonstrates surface exposure. In contrast, Nterminal regions that are inaccessible to trypsin are presumed to be translocation intermediates, or misfolded proteins in the cytoplasm.

The band corresponding to the MATE fusion protein was significantly reduced after trypsin treatment (FIG. 2). This is strong evidence that the passenger was exposed to the surface. The OmpA band at 37 kDa was unaffected by protease treatment, ensuring that the protease digestion did not affected membrane integrity. Overall, the data confirms that GFP was transported to the cell surface with high efficiency using the MATE system.

EXAMPLE 3

Secretion of a Recombinant Protein into the Medium using the MATE System

In this example, the MATE system utilises the OmpT protease to enable the secretion of a fulllength protein.

In this example, we show that GFP is secreted into the growth media after when expressed in OmpT positive strains using the MATE system.

Materials and Methods

pMATE-MT006 was transformed into E. coli UT2300 [F⁻, ara-14, leuB6, secA6, lacY1, proC14, tsx-67, entA403, trpE38, rfbD1, rpsL109(Str), xyl-5, mtl-1, thi-1] and its OmpT deficient derivate E. coli UT5600 [F⁻, ara-14, leuB6, secA6, lacY1, proC14, tsx-67, Δ(ompT-fepC)266, entA403, trpE38, rfbD1, rpsL109(Str^(r)), xyl-5, mtl-1, thi-1] (Grodberg and Dunn, 1988). 40 ml of LB medium was inoculated with 0.4 ml of an overnight culture, and grown until OD₆₀₀ 0.5 at 37° C., 200 rpm. 1 mM of IPTG was added, and the cells were incubated for another 1.5 hours. Cells were then removed by centrifugation (3750 g, 30 min, 4° C.) followed by vacuum filtration (0.45 μm HVLP membrane, Millipore).

Proteins secreted into the LB media were concentrated by TCA/Acetone precipitation. TCA (80% w/v) was added to the 40 ml of LB medium to a final concentration of 8.5%. The sample was then incubated for 1 h at 4° C., followed by centrifugation (3750 g, 30 min, 4° C.). The supernatant was then discarded except for a small amount (3 ml) which was used to resuspend the pellet and transfer to 1.5 ml tubes. After centrifugation at 18 000 g for 30 min, the pellet was resuspended in 1 ml of icecold acetone and followed by centrifugation. The pellet was then resuspended in icecold acetone in water (80% v/v) followed by centrifugation. The pellet was then dried for 20 min on a 37° C. heating block, and resuspended in 200 μl of 1×SDS sample buffer containing 100 mM of dithiothreitol (DTT). Proteins were dissolved by heating at 96° C. for 50 min with vigorous vortexing.

Proteins were separated by 12.5% SDS-PAGE and proteins visualised with Coomassie Brilliant Blue R250. Western blotting was conducted using a 1° antibody against GFP (rabbit polyclonal anti-GFP, #GTX26556, GeneTex) and a 2° goat anti-rabbit IgG antibody, coupled with horseradish peroxidase (#W401B, Promega). After SDS-PAGE, proteins were transferred onto a PVDF membrane using standard electroblotting techniques (Mini-trans blot, Bio-Rad). All solutions for Western blotting were based on Tris/Cl buffered saline (TBS, pH 7.4). The membrane was blocked for 1 h with 5% milk powder (blotting grade, Roth). It was then incubated for overnight in a 1:1000 dilution of 1° antibody, at 4° C. while shaking. The membrane was then washed with 0.1% Tween in TBS and incubated for 1.5 hours with a 1:6000 dilution of 2° antibody. Horseradish peroxidase activity was detected with luminol reagent (sc-2048, SantaCruz Biotechnology, USA) and a chemiluminescence imager (Chemocam, Intas).

Results and Discussion

To facilitate secretion via OmpT cleavage, we inserted an artificial OmpT cleavage site (Ala-Arg-Arg-Ala) into the MATE autotransporter in between the passenger and the linker. Because the EhaA autotransporter region has never before been used for surface display of a recombinant passenger, we were unsure whether OmpT would facilitate cleavage, and we were also unsure if the autotransporter domain would facilitate self-proteolysis as seen for autotransporters in the SPATE family.

To test if this artificial OmpT cleavage site could allow protein secretion, we transferred the plasmid pMATE-MT006 into OmpT positive strain UT2300 and looked for the presence of GFP in the growth media. Western blotting clearly showed the presence of GFP in the growth media of E. coli UT2300 pMATE-MT006 (FIG. 3). In contrast, we did not detect any GFP in the growth media after expression in the OmpT deficient derivate strain E. coli UT5600. The GFP visualised by Western blotting was visible as a single band with an apparent molecular weight of ˜30 kDa. The size of the GFP fragment detected by Western blotting strongly suggests that OmpT indeed cleaved at the artificial Ala-Arg-Arg-Ala site within the fusion protein. We did not see this band after SDS-PAGE followed by Coomassie staining, suggesting the amount of secreted protein was low. Nevertheless, we confirmed the ability to secrete proteins using this system, which should allow purification of the 6×His containing passengers, followed by cleavage of the 6×His region with Factor Xa.

When testing a new autotransporter system, it is important to determine if the surface displayed passengers are naturally released into the media by self-proteolysis. Self-proteolysis would reduce the amount of protein at the surface, and therefore reduce efficiency in biocatalysis or screening. In the MATE system we found no evidence that the passengers are released into the growth media by self-proteolysis. After surface expression in an OmpT negative strain (E. coli UT5600), GFP could be detected clearly in the outer membrane (see FIG. 2) but not in the growth media (FIG. 3). This reaffirms the efficiency of the MATE system for surface display in OmpT negative strains of E. coli. Since we did not detect any alternative OmpT fragments, it might be possible that the EhaA autotransporter linker does not contain any natural OmpT cleavage sites. This might allow the efficient surface expression using the MATE system in OmpT positive strains of E. coli.

Overall, our experiments show the feasibility of OmpT mediated secretion of recombinant passengers using the MATE system. OmpT did not cleave the passenger protein GFP, despite the presence of a typical OmpT recognition site within the primary sequence. We show for the first time that an artificial OmpT cleavage site can be used for the release of surface displayed passengers. In comparison to SPATE-like autoproteolytic cleavage, release by OmpT has the advantage that the same plasmid can be used for both surface display and secretion by simply varying the expression strain. This might have advantages in high-throughput approaches for protein expression and screening.

EXAMPLE 4

Secretion and Purification of Functional mCherry Protein using the MATE System in E. coli

Introduction

In this example, we show that mCherry is secreted into the growth media after expression in OmpT positive strains using the MATE system. The outer membrane protein T (OmpT) of E. coli is a surface membrane serine protease and is the prototypical member of the omptin family of gram-negative bacteria (Mangel, Toledo et al. 1994). Recombinant passengers have previously been shown to be secreted into the media, after surface display using autotransporters in OmpT containing E. coli strains. In all previous cases, an OmpT cleavage site was found within the autotransporter linker region. In our case it was unknown whether OmpT would facilitate cleavage, as the autotransporter within the MATE system (EhaA) has not been previously tested for the recombinant expression of passenger proteins. For this reason, we inserted an artificial OmpT cleavage site (Ala-Arg-Arg-Ala) to the Cterminal region of the recombinant passenger. Sevastsyanovich et al. already described the secretion of red fluorescent protein (RFP) mCherry by utilization of a serine protease autotransporter of the Enterobacteriaceae (SPATEs, Sevastsyanovich et al. 2012). In comparison to the cleavage procedure of the passenger domain of SPATES, the passenger of the MATE system is released by the endogenous protease OmpT by recognition of the artificial OmpT cleavage site within the plasmid.

Materials and Methods

Construction of pMATE-SI015

A ligation independent cloning was used for construction of pMATE-SI015

(In-Fusion Eco-Dry Kit, Clontech). The nucleotide sequence of pMATE-SI015 is described by SEQ ID NO:13 (FIG. 24). The autotransporter fusion protein encoded by pMATE-SI015 is described by SEQ ID NO:14. Therefore the pMATE-MT004 plasmid backbone was amplified with PCR using the primers S1020 (GCTCGTCGTGCTATTGAGGGCCGCATCCC) and PQ019

(ATGATGGTGATGGTGGTGGGTGATGTTCTG). The gene encoding mCherry was amplified using the primers SI021 (AATAGCACGACGAGCcttgtacagctcgtccatgccgccggtgg) and PQ024 (CACCATCACCATCATATGGTGAGCAAGGGCGAGGAGGATAACATG), which contained a 15 by overlap to the PCR product of the pMATE-MT004 plasmid backbone, as required for InFusion cloning. The two PCR Products were joined to form the plasmid pMATE-SI015 using standard In-Fusion techniques (Eco-Dry kit, Clontech) and transformed into E. coli Stellar chemically competent cells.

Bacterial Strains and Purification of mCherry

pMATE-SI015 was transformed into E. coli UT5600 and its OmpT positive parent strain E. coli UT2300 (Grodberg and Dunn, 1988).

800 ml of LB medium was inoculated with 8 ml of an overnight culture, and grown until OD₆₀₀ 0.5 at 37° C., 200 rpm. 1 mM of IPTG was added, and the cells were incubated for 24 hours. Cells were then removed by centrifugation (10,000 g, 20 min, 4° C.).

Proteins secreted into the LB media were purified via the 6×His epitope present in the secreted heterologous mCherry passenger. Therefore the obtained supernatant was loaded on a Ni-NTA column, which was before equilibrated with 10 column volumes of binding buffer containing 20 mM sodium phosphate, 0.5 M sodium chloride and 20 mM imidazole (pH 7.0). The column was washed once with 10 column volumes binding buffer and with 30 ml washing buffer, containing 20 mM sodium phosphate, 0.5 M sodium chloride and 100 mM imidazole (pH 7.0). Elution of the protein was carried out by addition of three times 2 ml Elution buffer containing 20 mM sodium phosphate, 0.5 M sodium chloride and 500 mM imidazole (pH 7.0). The eluted protein fractions were concentrated in microcon centrifugal filter devices with a cut-off size of 10 kDA (Merck Millipore). All steps were carried out at 4° C.

The concentrated protein was resuspended in 40 μl of 100 mM sodium phosphate buffer pH 7.0 plus 40 μl of 2×SDS sample buffer containing 200 mM of dithiothreitol (DTT). Protein samples were heated at 96° C. for 10 min.

Equal amounts of protein samples were analysed by 10% SDS-PAGE and visualised by Coomassie stain.

Results and Discussion

The outer membrane protein T (OmpT) of E. coli is a surface membrane serine protease and is the prototypical member of the omptin family of gram-negative bacteria (Mangel, Toledo et al. 1994).

As described for the secretion of GFP using the MATE system in example 3a), we also tested the artificial OmpT cleavage site for the secretion of monomeric red flourescent protein (RFP) mCherry. Therefore we transferred the plasmid pMATE-SI015 into OmpT positive strain E. coli UT2300 and looked for the presence of mCherry in the growth media.

Supernatants from cultures of both E. coli UT2300 and E. coli UT5600 showed no visible difference. We then attempted to purify mCherry from the supernatant using a Ni-NTA column. After applying the supernatant to the Ni-NTA column and elution with 500 mM imidazole the sample of OmpT-positive strain E. coli UT2300 was indeed pink. In constrast, repeating this procedure with the supernatant from OmpT-negative cells resulted in an eluate which was clear. This suggests that mCherry was indeed displayed at the surface and then released by the OmpT protease in the OmpT (FIG. 4).

The putative mCherry visualised by SDS-PAGE and subsequent Coomassie staining was visible as a single band with an apparent molecular weight of ˜30 kDa. In contrast, after expression and purification of the 6×His proteins from the OmpT negative derivate strain E. coli UT5600 we did not detect any RFP in the growth media, suggesting by the absence of a band at the same molecular weight as it is the case for the OmpT positive strain (FIG. 5).

The presence of a band with an apparent molecular weight of ˜100 kDa for both, the OmpT positive and OmpT negative strain protein samples, suggests the co-purification of the full autotransporter fusion protein either after cell lysis or by occurrence of vesicles containing the autotransporter fusion protein (FIG. 5).

The size of the band from the eluate from E. coli UT2300 detected by SDS-PAGE with subsequent Coomassie stain strongly suggests that OmpT indeed cleaved at the artificial Ala-Arg-Arg-Ala site within the fusion protein and released the putative mCherry passenger. Overall, our experiments show the feasibility of OmpT mediated secretion of recombinant passengers using the MATE system. As it is described in Example 3a) for the GFP passenger, we did not detect any undesired cleavage of the mCherry passenger by OmpT. This example gives a further proof that the artificial OmpT cleavage site can be used for the release of surface displayed passengers. The OmpT mediated release has the advantage that the same plasmid can be used for both surface display and secretion. This allows greater flexibility than self-proteolysis, where it would be necessary to construct a second plasmid for surface display in which the residues necessary for self-proteolysis are removed.

EXAMPLE 5

Release of Recombinant Passenger using Factor Xa, after Surface Display using the MATE System

In this example, the MATE system is shown to enable the release of a fulllength protein using the specific protease Factor Xa.

We showed earlier that the MATE system could be used for the constitutive secretion of a recombinant passenger in OmpT positive strains (Example 3). The secretome of E. coli is known to be relatively simple, reducing the costs associated with purification in comparison to intracellularly expressed proteins. However protein purification is still required to separate the recombinant protein of interest from other secreted proteins. For high-throughput screening, it would be beneficial to obtain small amounts of the protein of interest at higher purity. This could be achieved by first displaying the protein of interest on the cell surface, washing the cells in buffer, and then releasing the protein of interest into the buffer by the activity of a specific protease. The protein of interest can then be immediately assayed for functional activity. Optimally, the protease used to release the passenger is highly specific. In this example, we show that the protease Factor Xa can successfully release passengers displayed on the cell surface with the MATE system.

E. coli UT5600 cells containing pMATE-MT006 were grown in an overnight culture in LB medium. 20 ml of LB medium was inoculated with 0.5 ml of overnight culture, and grown at 37° C., 200 rpm to OD₆₀₀ of 0.5. Protein expression was induced by the addition of 1 mM IPTG, and the cells were harvested after 1.5 hour. Cells were washed in buffer consisting of 100 mM Tris/Cl pH 8, 50 mM NaCl, 1 mM CaCl₂, and incubated for 16 h with 100 μg·ml⁻¹ of bovine Factor Xa protease (#33233, QIAGEN). Cells were removed by centrifugation at 14 000 g, and proteins within the supernatant were concentrated by TCA precipitation (final TCA concentration of 8%). Concentrated proteins from the supernatant were separated by 12.5% SDS-PAGE. Western blots were performed with polyclonal rabbit anti-GFP and horseradish peroxidase-coupled anti-rabbit IgG as described in Example 3.

Soluble GFP (˜28 kDa) was detected in the supernatant after Factor Xa treatment of E. coli pMATE-MT006 (FIG. 6). No significant amounts of GFP were released without the addition of Factor Xa, suggesting that the MATE autotransporter does not undergo self-proteolysis as seen in SPATE autotransporters. The addition of the Factor Xa cleavage site after the passenger therefore allows the selective release of passengers from the cell surface.

In combination with the 6×His on the Nterminal region of the passenger, the selective release in the MATE system allows the rapid purification of proteins. As shown here, the cells can be washed before the release of the passenger into a buffer. This yields a purer form of the protein in comparison to a constitutive secretion system such as auto-proteolysis, where the passenger is released into the growth media and must be separated from other secreted proteins.

The Factor Xa mediated release also allows a more rapid protease-accessibility assay in the MATE system. Usually a nonspecific protease such as trypsin or proteinase K is used, whereby the incubation time must be optimised for each system. Proteins transported to the surface with Autodisplay have also been released with purified Iga1 protease (Klauser 1992), however this enzyme is not commercially available and the mode of action is not extensively researched. Only recently has this been attempted with a commercially available, highly specific protease (Nla-TEV, Ko et al. 2012). Here, we show the detection of the passenger in the supernatant after incubation of the cells with Factor Xa, a rapid and simple method of proving surface display, without the need for antibodies or extensive optimisation of protease digestion. When optimised, it may be also possible to detect the decrease in cell-associated passenger after Factor Xa treatment. This technique allows rapid and simple protein purification, and may be compatible with highthroughput screening approaches for protein improvement or inhibitor detection.

EXAMPLE 6

Functional Display of B. gladioli EstA Esterase on the Surface of E. coli with the MATE System

In this example the EstA catalytic domain was functionally expressed on the surface of E. coli using the new MATE system.

Plasmid Construction

The plasmid pMATE-SI005 (FIG. 20, SEQ ID NO:7) was designed for the surface expression of proteins using the MATE system in a broad range of Gram negative bacteria. To create the plasmid, PCR products from three plasmids were combined using ligation independent cloning methods (In-Fusion, Clontech, USA). These fragments included the MATE autotransporter from pMATE-MT004, the rep and kanamycin resistance genes (aph) from pBBR1MCS-2 (Kovach et al. 1995), and two fragments from pBAD/gIII. The first fragment from pBAD/gIII contained the araBAD promoter and araC gene. The second fragment from pBAD/glIl contained the rrnB transcription terminator.

The fusion protein encoded by pMATE-SI005 (SEQ ID NO:8) included a CtxB signal peptide, a small peptide passenger (6×His), a multiple cloning site, OmpT and Factor Xa cleavage sites and a PEYFK epitope. The Cterminus contains the EhaA autotransporter linker and n-barrel.

For the surface display of a functional esterase, the coding region of the catalytic domain of estA from Burkholderia gladioli was excised from pES01 (Schultheiss et al. 2002) via Xhol and Kpnl and subsequently inserted in pMATE-SI005 to create pMATE-SI010 (FIG. 7, and see plasmid map in FIG. 22).

We conducted activity tests to determine if EstA catalytic domain was expressed in a functional form. For this purpose cells of E. coli UT5600 pMATE-SI010 and E. coli BL21(DE3) pES01 were cultivated at 30° C. E. coli UT5600 without any plasmid served as a control. E. coli UT5600 cells were induced with 0.5% (w/v) L-arabinose for 2 hours. Cells of E. coli BL21 (DE3) pES01 were also cultivated for 2 hours. Since pES01 plasmid encodes a promoter facilitating a constitutive gene expression there was no need for any induction. Photometric activity assays were performed with pnitrophenyl acetate as a model esterase substrate. To obtain standard conditions OD₆₀₀ was adjusted to 0.2 for every measurement. In comparison to pES01 the MATE system gives vastly improved activity of surface-displayed esterase (FIG. 8).

EXAMPLE 7

Functional Surface Display of EstA Catalytic Domain in Salmonella enterica using the MATE System

In this example the MATE system was used for expression of the estA catalytic domain in a second non E. coli strain, namely S. enterica serovar Typhimurium. Autotransporter-mediated surface display has been previously conducted in Salmonella strains (van Gerven et al. 2009), which are genetically closely related to E. coli. However this provides the first confirmation that the vector is compatible in non E. coli strains.

The broad host range plasmid pMATE-SI010 (FIG. 22, SEQ ID NO:9), encoding the new autotransporter EhaA and the catalytic domain of EstA (SEQ ID NO:10) was transferred to cells of S. enterica. Protein expression was induced with 0.5% (w/v) L-arabinose for two hours at 30° C., 200 rpm. To find out whether the EstA catalytic domain of the fusion protein was indeed exposed at the cell surface, proteinase K was added the whole cells.

Proteinase K is too large to enter the cell envelope of S. enterica. This means, if the EstA catalytic domain is degraded by proteinase K, it must be accessible at the cell surface.

Activity assays with pnitrophenyl acetate as a substrate were performed with either proteinase K treated or untreated whole cells containing S. enterica pMATE-SI010. Protein expression was induced for 2 h with 0.5% L-arabinose at 30° C. The final OD₆₀₀ in the assay was adjusted to 0.2. We used S. enterica, without any plasmid as a negative control. Cells of S. enterica pMATE-SI010 exhibited similar activity in comparison to E. coli cells harbouring pMATE-SI010 plasmid (FIG. 9).

The decrease in esterase activity at the surface after proteinase K treatment confirms that the estA catalytic domain was exposed to the surface. This confirmed that the MATE system functions not only in E. coli, but also in a closely related non E. coli species. The use of the MATE system in S. enterica may help in the development of vaccines. To our knowledge, this is the first time that surface display of a recombinant protein with an autotransporter has been successfully achieved in a Salmonella species.

EXAMPLE 8

Functional Surface Display of EstA Catalytic Domain in Pseudomonas putida KT2440 with the MATE System

In this example the MATE system was used for the functional expression of and enzyme in a Gram negative species more distantly related to E. coli.

Functional surface display of estA in E. coli was carried out as described in Example 4. The broad host range plasmid pMATE-SI010, encoding the catalytic domain of the esterase EstA, was transferred to cells of P. putida KT2440 using standard chemical transformation techniques.

Functional activity was first tested using continuous esterase microplate assays. Esterase activity in P. putida cells containing pMATE-SI010 was significantly higher than cells containing the control plasmid, pMATE-SI005 (FIG. 10). When adjusted to an equal OD600, activity was higher in E. coli than in P. putida.

To confirm that this functional activity was at the cell surface, the esterase microplate assays were conducted with whole cells after incubation with proteinase K. Proteinase K is too large to cross the outer membrane. The loss of activity in whole cells after addition of proteinase K givesstrong evidence that the esterase is located at the cell surface. Using pnitrophenyl acetate as a substrate, activity assays were performed with either proteinase K treated or untreated whole cells of P. putida pMATE-SI010.

P. putida cells expressing an esterase at the surface using the MATE system completely lost their esterase activity after treatment with proteinase K (FIG. 11). In comparison, approximately half of the esterase activity was lost in E. coli cells under the same conditions. This implies that the EstA esterase had a better accessibility to the protease in P. putida than in E. coli.

We conducted flow cytometry analysis to confirm that the fusion protein containing the EstA fragment was exposed at the surface. The results are described in FIG. 12. P. putida KT2440 cells were grown in LB medium to an early exponential growth phase (OD₆₀₀ of 0.5). After cell harvest and resuspension in phosphate buffered saline (PBS), expression of EstA was induced for 18 hours by adding Larabinose to a final concentration of 0.5%. Cells were then incubated for 18 hours at 30° C., 200 rpm. Cells were harvested by centrifugation, washed twice with PBS and resuspended to a final OD₆₀₀ of 0.4. 1 ml of the cell suspension was centrifuged (90 s at 13.400 g), resuspended in 100 μL of a solution that contained a monoclonal mouse anti-6×His antibody (20 μg·ml⁻¹ in PBS, Thermo Scientific) and incubated for 60 min at 22° C. Cells were then washed twice with 500 μL of PBS. The second incubation step was conducted in the dark (60 min, 22° C.) using 100 μL of a rabbit antimouse IgG antibody conjugated with fluorescein (20 μg·ml⁻¹ in PBS). After washing twice in PBS, the cell pellet was resuspended in 1 ml of PBS for analysis. Samples were then analysed using a BD FacsAria III Cell Sorter (BD Biosciences, Heidelberg, Germany) at an excitation wavelength of 488 nm.

The fully processed, surface-displayed EstA fusion protein was predicted to have an N-terminus consisting of a 6×His affinity tag, the EstA fragment and MATE autotransporter domain. Using an antibody against the 6×His epitope, approximately 50% of the cells were found in a population with high fluorescence and could be judged to contain the fusion protein at the surface. To determine whether non-stained cells represented incomplete surface expression, or simply sub-optimal antibody binding, we conducted further analyses of cellular and outer membrane proteins.

As with many other esterases, EstA can be refolded after SDS-PAGE and functional activity measured using an ingel activity stain (Schultheiss et al. 2002). Briefly, proteins were heated for 30 min in loading buffer that contained DTT, and separated by 10% SDS-PAGE as per standard techniques (Laemmli 1970). The gels were then incubated in buffer containing 2.5% Triton-X100 until the esterase had refolded into an active form. The gels were then stained for esterase activity using 1naphthyl acetate as a substrate and Fast Blue RR as a conjugate dye.

In the analysis of whole cells expressing EstA at the surface, SDS-PAGE followed by esterase staining revealed a band at approximately 105 kDa in both E. coli and P. putida (FIG. 13B). The expected size of the fusion protein after cleavage of the signal peptide was 91.2 kDa. As expected, this band was not visible after Coomassie staining, as it comprised only a small proportion of total cellular protein. A single esterase band was visible in P. putida, however esterase activity at the apparent molecular weight of ˜38 kDa and ˜60 kDa were also visible in E. coli.

A further SDS-PAGE analysis was conducted to confirm the surface exposure of the EstA esterase in P. putida, when expressed using the MATE system. To find out whether the EstA catalytic domain of the fusion protein was indeed exposed at the cell surface, proteinase K was added the whole cells containing pMATE-SI010 after protein induction with 0.5% (w/v) L-arabinose for two hours at 30° C. Proteinase K is too large of a molecule to enter the cell envelope of E. coli, the decrease in bands associated with the fusion protein after Coomassie or esterase staining indicates the surface exposure of the esterase. SDS-PAGE with Coomassie stain allows a further control: OmpA has a Nterminal extension in the periplasm that is proteinase K sensitive, therefore the detection of intact OmpA indicates that the protease has not entered the periplasm due to cell leakiness.

After isolation of membrane proteins from P. putida, the EstAautotransporter fusion protein was visible after SDS-PAGE with both Coomassie and esterase staining (FIG. 14). As we showed previously for the GFP fusion protein, the apparent molecular weight (MW) of the EstA-autotransporter fusion protein after SDS-PAGE (˜105 kDa) was higher than the molecular weight predicted from the primary sequence after accounting for signal peptide cleavage (91.2 kDa). It is not unusual for βbarrel proteins to show an altered apparent molecular weight after SDS-PAGE, as they remain partially folded and in some cases catalytically active in conditions that are strongly denaturing for other proteins. There was no difference in the apparent MW between the fusion protein expressed in E. coli and P. putida.

Confirming the results from microplate assays (FIG. 11), proteinase K treatment of P. putida cells expressing EstA at the surface completely removed all esterase activity, as judged by the disappearance of the esterase band after SDS-PAGE (FIG. 14). In contrast, protease treatment gave only a partial decrease in the intensity of the esterase band from E. coli. This strongly supports the results from microplate assays (FIG. 11), where only a proportion of the E. coli esterase activity was susceptible to external protease treatment (FIG. 11). Overall, this strongly suggests that a proportion of the esterase molecules in E. coli were intracellular, and therefore inaccessible to the protease. This hypothesis is supported by the undesired 38 kDa and 60 kDa esterase fragments seen in the analysis of E. coli whole cells. These fragments cannot be located at the cell surface, because they are not visible in the analysis of outer membrane proteins (FIG. 14).

In comparison to E. coli, the vast majority of esterase activity in P. putida pMATE-SI010 was protease accessible. This could suggest that P. putida has unexpected advantages for use with surface display. Another surprising benefit within P. putida was the absence of of the undesired intracellular fragments, which may indicate a small amount of the fusion protein is improperly folded in E. coli. These factors suggest that P. putida is highly suitable to screening of surface displayed enzymes or fluorescent molecules where intracellular activity is detrimental.

When we directly compared the surface-exposed (i.e. protease accessible) esterase activity of E. coli and P. putida, the overall activity in E. coli was higher (FIG. 15). Nevertheless, P. putida clearly showed some advantages over E. coli for surface display—not only is the strain chemical resistant and compatible with industrial processes, but a higher proportion of the passenger was found at the surface. This highlights the advantage of the MATE system, whereby the surface display of recombinant proteins can be rapidly tested in a broad range of bacterial hosts.

EXAMPLE 9

Functional Surface Display of B. gladioli Esterase EstA Catalytic Domain in Zymomonas mobilis with the MATE System

Introduction

Zymomonas mobilis is of big interest for the commercial production of biomass-derived ethanol as it produces ethanol with high yields and has a higher growth rate than the presently used Sacharomyces cerevisiae. The exposure of recombinant proteins on the surface of Z. mobilis offers considerable advantages in the industrial application of this organism. For instance, the surface display of cellulose degrading enzymes (cellulases) on Z. mobilis cells can provide a whole-cell catalytic system which is able to breakdown cellulose and ferment the formed monomeric sugars to ethanol in a single step. As cellulose cannot penetrate the cell membrane, elaborate and expensive enzyme purification steps become necessary when the cellulases are expressed intracellularly. Surface exposed cellulases could circumvent these difficulties as they have direct access to their substrates and do not have to be extracted from the cells prior to their use. A further benefit of a whole-cell catalyst is given by its reusability, since bacterial cells can easily be separated from a reaction mixture by centrifugation.

To our knowledge no surface display system for Z. mobilis has been established so far. This example presents the functional expression of B. gladioli esterase EstA catalytic domain on the surface of Z. mobilis using the new MATE system.

Materials and Methods

Construction of Plasmid pMATE-SI012 and Transfer to Z. mobilis

For the construction of pMATE-SI012 (FIG. 23, SEQ ID NO:11) genes encoding the estA catalytic domain were excised from plasmid pMATE-SI010 via Xhol/Kpnl restriction endonucleases and ligated into plasmid pMATE-SI009 (FIG. 21). This plasmid contains a mob gene which is necessary for plasmid replication in Z. mobilis. pMATE-SI012 was transferred to cells of Z. mobilis ATCC 29191 using a standard electroporation procedure. The fusion protein is described in SEQ ID NO:12).

Cultivation of Z. mobilis and Induction of Protein Expression

200 mL of ZM Medium (10 g/L bacto peptone, 10 g/L yeast extract, 20 g/L glucose; Roth, Germany, when needed kanamycine 150 mg/mL; Sigma Aldrich, USA) were inoculated with an overnight-culture of Z. mobilis (1:1000 dilution) and incubated at 30° C., 60 rpm until the cultures reached an OD578 of 0.6. Induction of protein expression was performed by incubation with 0.2% (w/v) L-arabinose (Roth, Germany) in ZM Medium for two hours at 30° C., 60 rpm.

Trypsin Digestion

Z. mobilis cells were harvested by centrifugation and resuspended in 0.2 mol/L Tris-HCl pH 8.0. Porcine pancreatic trypsin was added to a final concentration of 2.5% and incubated for one hour at 37° C., 200 rpm. Digestion was stopped by washing three times with 10% fetal calf serum (FCS) in 0.2 mol/L Tris-HCl pH 8.0.

Outer Membrane Protein Isolation

Outer membrane protein isolation was carried out according to a modified protocol of Hantke (Hantke 1981, Jose and von Schwichow, 2004).

Protein Separation

Proteins were heated for 40 min at 95° C. in two-fold sample buffer (100 mM Tris-HCl, pH 6.8 containing 4% sodium dodecyl sulfate, 0.2% bromophenole blue, 20% glycerol and 30 mg dithiothreitol) and separated by means of sodium-dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in a 10% resolving gel (Laemmli 1970). Proteins were then stained either with Coomassie Brilliant Blue or esterase activity stain (see below). Pictures were taken using an Intas Gel iX Imager (Intas, Germany).

Western Blot

Standard blotting techniques were used to transfer proteins from polyacrylamide-gels to nitrocellulose membranes (Mini-trans blot, Bio-Rad, USA). The membranes were blocked with 3% bovine serum albumine (BSA) in phosphate-buffered saline (PBS, pH 7.4) and incubated with mouse anti-6×His mAb IgG1 (Dianova, Germany) in PBS (1:1000 dilution). Subsequently the membranes were washed three times with PBS and incubated with horseradish peroxidase-conjugated anti-mouse IgG (Antibodies-online, USA) in PBS (1:5000 dilution). The membranes were then washed twice with PBS and treated with Pierce enhanced chemiluminescent (ECL) western blotting substrate (Thermo Scientific, USA). Pictures were taken using an Intas ChemoCam Imager (Intas, Germany).

Esterase Activity Staining

Esterase activity staining was carried out using a modified protocol of Schultheiss et al. (Schultheiss 2002). For the renaturation of the esterase SDS was removed by incubation of the polyacrylamide gels in 10 mmol/L Tris-HCl, pH 7.5 containing 2.5% Triton-X100 (AppliChem, Germany) for three hours. The gels were then stained for esterase activity with 10 mmol/L

Tris-HCl, pH 7.5 containing 0.1% (w/v) FastBlueRR (Sigma Aldrich, USA) and 2% (v/v) α-naphtyl-acetate-solution (1% w/v in 50% v/v acetone; Sigma Aldrich, USA).

Results

SDS-PAGE Coomassie Staining

Outer membrane proteins of Z. mobilis cells without plasmid pMATE-SI012, cells with plasmid and cells with plasmid and aforegoing trypsin treatment were isolated and separated by SDS-PAGE. Coomassie staining of proteins isolated from cells with plasmid did not show any additional bands assignable to the EstA-autotransporter fusion protein. This is attributed to the small proportion of fusion protein in relation to the total outer membrane protein amount. Coomassie staining of proteins isolated from cells that were treated with trypsin prior to the outer membrane protein isolation revealed the disappearing of most protein bands except of four bands, which are visible at apparent molecular weights of <25 kDa (a), 25-35 kDa (b) and 35-40 kDa (c and d) (see FIG. 16A). The proteins within these bands seem to be resistant to Trypsin degradation. We excised the bands from the gel and analysed them by LC-MS/MS in order to identify the proteins within each band. 15 to 17 different Z. mobilis proteins were identified in each band. It is not possible to obtain quantitative information, however, OmpA/MotB domain containing proteins (Uniprot accession number: I6YFM2) could be shown to be present in bands b, c and d. Both OmpA and MotB are known to be integral membrane components. As Trypsin is too large to penetrate the outer membrane and therefore digests only proteins that are located on the cell surface, this is good evidence that the outer membrane remained undamaged by trypsin treatment and that only exterior proteins were degraded.

Western Blot

Western Blot analysis of outer membrane protein isolates with anti-6×His antibodies showed the presence of the EstA-autotransporter fusion protein at an apparent molecular weight of about 105 kDa, which is slightly higher than expected from the primary sequence (91.2 kDa). This is not unusual for beta-barrel containing proteins as they are not completely denatured under normal SDS-PAGE conditions. Neither in the outer membrane protein isolates of Z. mobilis cells without plasmid nor in the protein isolates of cells with plasmid but without L-arabinose induction a band was visible. Protein isolates of cells which were treated with trypsin prior to protein isolation also did not show any band. The absence of the fusion protein in the outer membrane proteins of trypsin-digested cells confirms the actual exposure of the esterase on the surface of the Z. mobilis cells (see FIG. 16B).

Esterase Activity Stain

In-gel esterase activity staining revealed an activity band at about 105 kDa in outer membrane proteins of arabinose treated Z. mobilis cells with plasmid. This is consistent with the presented Western Blot results, which identified this band as the EstA-autotransporter fusion protein. Besides this band, three other esterase activity bands between 55 and 100 kDa were visible in the same protein isolate. It is believed that these activity bands are unspecific degradation products. Esterase activity bands could neither be detected in protein isolates of cells without plasmid nor in protein isolates of cells with plasmid but without arabinose induction. Outer membrane proteins of cells that were trypsin digested also showed no esterase activity at all (see FIG. 17). This strongly supports the assumption that EstA is functionally active on the surface of Z. mobilis cells

Conclusion

Besides the successful establishment of the MATE system in P. putida and S. enterica, these experiments confirm the proper functionining of the MATE system in a third Zymomonas mobilis, a natural ethanol producer.

EXAMPLE 10

Surface Display of an Active Endoglucanase Obtained from Bacillus subtilis on the Ethanologenic Bacteria Zymobacter palmae and Zymomonas mobilis using Maximized Autotransporter Expression (MATE) System

Introduction

The biocatalytic conversion of cellulose to ethanol as a doorway to alternative fuel production is to date limited by the absence of a catalytic system applicable to economic large scale processes. The biocatalyst of choice combines the ability to break down cellulose and simultaneously ferment the released sugars to ethanol. The high growth and ethanol production rates of the Gram-negative bacteria Zymobacter palmae and Zymomonas mobilis make them promising host organisms for such an approach. Since they cannot degrade cellulose naturally, it is necessary to add extracellular (i.e. secreted or cell-bound) endoglucanase, exoglucanase and beta-glucosidase functionalities to these organisms. In this example we describe the expression of a recombinant fusion protein consisting of an endoglucanase obtained from Bacillus subtilis and the maximized autotransporter expression (MATE) translocation unit in Z. palmae and Z. mobilis, resulting in the exposure of functional active endoglucanase on the cell surface of of both species.

Construction of Plasmid pMATE-PT004

The plasmid pMATE-PT004 was constructed based on the previously described pMATE-SI010 (SEQ ID NO:9). Plasmid pMATE-SI010 encodes a fusion protein consisting of an N-terminal CtxB signal peptide, Burkholderia gladioli esterase EstA catalytic domain and the C-terminal EhaA autotransporter domain. The expression of the fusion protein is controlled by an arabinose inducible promoter (araBAD). For detection and purification purposes, 6×His and PEYFK-epitopes are incorporated N- and C-terminal of the esterase domain, respectively. The plasmid encodes a Kanamycin resistance gene for transformant selection (FIG. 22).

The esterase EstA catalytic domain of pMATE-SI010 was removed by restriction enzyme digestion with Xhol and Kpnl, while the endoglucanase domain was obtained by an identical restriction enzyme digestion of pMATE-endoglucanase and inserted into pMATE-SI010 to generate pMATE-PT004. Furthermore, a mob gene was inserted into pMATE-PT004 using ligation independent cloning methods (In-Fusion Cloning Kit HD, Clontech, USA) to enable plasmid replication in Z. mobilis and Z. palmae (FIG. 26).

Cultivation and Transformation of used Bacterial Strains

Z. mobilis (DSM No. 3580) was cultured at 30° C., 80 rpm in culture medium containing 10 g/L bacto peptone, 10 g/L yeast extract and 20 g/L D-glucose. Z. palmae (DSM No. 10491) was cultured at 30° C., 200 rpm in growth medium containing 10 g/L yeast extract, 2 g/L potassium dihydrogen phosphate and 5 g/L sodium chloride, adjusted to pH=6.0. Insertion of plasmids was conducted according to standard electroporation procedure, electroporated cells were regenerated in culture medium for 6 h (Z. mobilis) and 2 h (Z. palmae), before transferring them to Kanamycin selection plates.

Sodiumdodecylsulfate Polyacrylamide Gel Electrophoresis (SDS PAGE)

Overnight cultures of Z. mobilis/Z. palmae containing pMATE-PT004 were used to inoculate 100 ml of the respective culture medium (1:100 dilution), and were incubated until the cultures reached OD₅₇₈ of 0.5. Protein expression was induced by the addition of 0.2% L-arabinose, and the cells harvested one hour after induction. Outer membrane proteins were prepared according to the rapid isolation protocol of Hantke (Hantke 1981) with modifications as described previously (Jose and von Schwichow, 2004).

Proteins were separated with 12.5% sodium-dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) (Laemmli 1970). Proteins were stained with Coomassie Brilliant Blue R250.

Fluorescence-Activated Cell Sorting (FACS)

Cells were cultivated as described above and adjusted to an OD₅₇₈ of 0.4. After washing three times with cold, particle-free PBS, cells were resuspended in PBS containing 1° antibody against 6×His (THE HisTag, mouse, GenScript, USA) in a dilution of 1:500. After 30 min incubation at 4° C., cells were washed three times with PBS and incubated 30 min with 2° antibody (goat anti-mouse IgG (H+L), Dylight 633 conjugated, Thermo Scientific Pierce Antibodies, Germany) at room temperature in the dark. Cells were washed again three times and cell fluorescence measured by FACS Aria III flow cytometer (BD Biosciences, USA) using a red laser for excitation at 633 nm and a 660/20 bandpass filter for detection.

Whole Cell CMC Hydrolysis Activity Assay

For the whole cell endoglucanase activity assay cells were cultivated as described above and adjusted to an OD of 25. Cells were incubated 10 min with 1% Carboxymethylcellulose (CMC) in sodium-citrate buffer, pH 6 at temperatures between 30° C. and 80° C. After cell removal, released reducing sugars were determined photometrically (absorption at 540 nm) using a modified 3,5-dinitrosalicylic (DNS) assay protocol of King et al. (2008). The absorption values of blank samples (buffer and substrate) were subtracted from the measured absorption values of the cell samples.

Expression of Fusion Protein

To confirm the expression of the endoglucanase fusion protein, outer membrane proteins of Z. palmae were isolated and separated by SDS-PAGE (FIG. 27). While in the outer membrane isolates of Z. palmae wildtype (lane 2) and non-induced Z. palmae pMATE-PT004 (lane 3) no band between 100 kDa and 130 kDa is visible, a strong band can be seen in the outer membrane isolate of induced Z. palmae pMATE-PT004 (lane 4) between 100 kDa and 130 kDa, which is consistent with the expected molecular weight of the endoglucanase fusion protein. To further check the localization of the protein on the cell surface, induced Z. palmae pMATE-PT004 cells were treated with trypsin prior to the outer membrane isolation (lane 5). Since trypsin is too large to penetrate the outer cell membrane, it can exclusively degrade surface-exposed proteins. The disappearance of the band assigned to the fusion protein in lane 5 suggests that the expressed protein is accessible to trypsin and therefore located on the cell surface.

Whole Cell CMC Hydrolysis Activity Assay

Z. palmae wildtype cells, Z. palmae cells with pMATE-esterase (negative controls) and cells with pMATE-PT004 were analysed in terms of their CMC hydrolysis activity. Induced Z. palmae cells containing the pMATE-PT004 plasmid show high CMC hydrolysis activity compared to negative controls.

CMC hydrolysis activity could be observed within a temperature range of 30° C. to 80° C. Non-induced cells with pMATE-PT004 show slightly higher activity than the negative controls, explained by the known effect of low basal protein expression level when using an arabinose promoter (FIG. 28).

Whole cell CMC hydrolysis activity assay was also performed with Z. mobilis wildtype and Z. mobilis pMATE-PT004 cells. Z. mobilis pMATE-PT004 cells showed high activity in a temperature range of 30° C. to 80° C. compared to Z. mobilis wild type cells (FIG. 29). Although an expression of the protein could not be confirmed by SDS-PAGE, the determined activity proves the existence of the enzyme on the cell surface.

Since CMC is a large polymer which is not able to pass the cell wall, neither by diffusion nor by active transport, the observed hydrolysis of CMC represents strong evidence for the localisation of the enzyme on the cell surface.

FACS

FACS-histogram of Z. palmae pMATE-PT004 cells expressing the endoglucanase fusion protein (FIG. 31) shows a total increase of cell fluorescence compared to Z. palmae wildtype cells (FIG. 30). As antibodies cannot penetrate the cell membrane and thus only bind to surface exposed epitopes, this experiment gives strong evidence that the endoglucanase is located on the surface of Z. palmae cells expressing the endoglucanase fusion protein. This supports the results of the described SDS-PAGE and activity assays.

Conclusion

We could successfully apply the MATE system to express a surface-displayed, active endoglucanase in the ethanologenic bacteria Zymomonas mobilis and Zymobacter palmae. This example illustrates the broad applicability of our invention to bacterial strains with prospective industrial use, in particular in combination with cellulose-degrading enzymes and ethanol-producing bacteria, forming a whole-cell biocatalyst for the production of second generation biofuels.

EXAMPLE 11

Functional Display of Bacterial Cellulases on the Surface of Pseudomonas putida with the pMATE System

In this example an endoglucanase obtained from Bacillus subtilis, an exoglucanase obtained from Clostridium thermocellum and a 3-glucosidase obtained from Clostridium thermocellum were functionally expressed on the surface of P. putida using the new pMATE system. Plasmids used for transformation of P. putida are described in FIGS. 38, 39 and 40.

SEQ ID NO:20 describes the nucleotide sequence of the autotransporter fusion gene encoded by pMATE-exoglucanase, for the surface display of an exoglucanase using the pMATE system.

SEQ ID NO:21 describes the polypeptide sequence of the autotransporter fusion protein encoded by pMATE-exoglucanase, for the surface display of an exoglucanase using the pMATE system.

SEQ ID NO:22 describes the nucleotide sequence of the autotransporter fusion gene encoded by pMATE-endoglucanase, for the surface display of an endoglucanase using the pMATE system.

SEQ ID NO:23 describes the polypeptide sequence of the autotransporter fusion protein encoded by pMATE-endoglucanase, for the surface display of an endoglucanase using the pMATE system.

SEQ ID NO:24 describes the nucleotide sequence of the autotransporter fusion gene encoded by pMATE-β-glucosidase, for the surface display of a β-glucosidase using the pMATE system.

SEQ ID NO:25 describes the polypeptide sequence of the autotransporter fusion protein encoded by pMATE-β-glucosidase, for the surface display of a β-glucosidase using the pMATE system.

The expression was tested by isolation of the outer membrane proteins and following separation by 10% SDS-PAGE (FIG. 32). Proteins were visualized with Coomassie Brilliant Blue R250. For this purpose cells were cultivated at 30° C. in LB medium until they reached an OD₅₇₈ of 0.5. Protein expression was induced with 0.2% (w/v) L-arabinose for 4 h at 30° C. P. putida without the pMATE-plasmid served as control. The exo- and endoglucanase fusion proteins were visible as a single band at the predicted size (ca. 147 kDa and 110 kDa, respectively after cleavage of the N-terminal signal peptide). As expected the fusion proteins were not visible from cells without induction of protein expression or control cells without plasmid. In case of the β-glucosidase, a very weak expression of the fusion protein was detected at the predicted size (ca. 109 kDa).

For activity assays cells were cultivated as described above. The exoglucanase activity was measured at pH 6 and 55° C. using 5 mM p-nitrophenyl-3-D-cellobioside as substrate. OD₅₇₈ were adjusted to 0.5. After different incubation times cells were removed by centrifugation and the liberated p-nitrophenol in the supernatant was detected colorimetrically at 400 nm in order to determine the hydrolysis rate. In comparison to control cells, P. putida cells, expressing the exoglucanase, show continuous p-nitrophenol release within 4 min (FIG. 33).

The endoglucanase activity was measured by determining reducing sugars released of enzyme reaction with 1% carboxymethylcellulose (CMC) at pH 6 and 55°. OD₅₇₈ were adjusted to 20. After cell removal reducing sugars were detected in the supernatant colorimetrically at 540 nm using the 3,5-dinitrosalicylic acid (DNS) assay, modified by King et al (2008). In comparison to control cells, endoglucanase expressing P. putida cells show CMC-hydrolysis (FIG. 34).

The β-glucosidase activity was measured at pH 6 and 55° C. using 5 mM p-nitrophenyl-β-D-glucopyranoside as substrate. OD₅₇₈ were adjusted to 20.

After different incubation times cells were removed by centrifugation and the liberated p-nitrophenol in the supernatant was detected colorimetrically at 400 nm in order to determine the hydrolysis rate. In comparison to control cells, P. putida cells, expressing the β-glucosidase, show p-nitrophenol within 10 min (FIG. 35).

These examples show that the pMATE system can be used for functional display of cellulases on the surface of P. putida.

The most common total cellulase activity assay is the filter paper assay

(FPA) using Whatman No 1 filter paper as the substrate, which was established and published by the International Union of Pure and Applied Chemistry (IUPAC) (Ghose 1987). To measure total pMATE-cellulase activity, exoglucanase, endoglucanase and β-glucosidase were mixed in equal parts. OD₅₇₈ of total cells were adjusted to 50. The FPA was performed at pH 6 and 55° C., modified by Xiao et al (2004). Reducing sugars, released by degradation of filter paper were detected in the supernatant colorimetrically at 540 nm using the 3,5-dinitrosalicylic acid (DNS) assay, modified by King et al (2008). In comparison to control cells, the mix of cellulase expressing P. putida cells show filter paper hydrolysis. Using glucose as standard the amount of released sugar equivalents (RSE) from filter paper after 4 days is 0.2 mg/ml (FIG. 36).

To determine the hydrolysis of a real lignocellulosic substrate by pMATE-cellulases, pretreated empty fruit punches (EFP) were used as substrate (2.5% dry weight). Exoglucanase, endoglucanase and β-glucosidase expressing cells were mixed in equal parts. OD₅₇₈ of total cells were adjusted to 20. The reaction was performed at pH 6 and 55° C. for 4 days. Reducing sugars (in particular glucose, cellubiose or/and cellulose-polysaccharide chains of variable length), released by EFB hydrolysis were detected in the supernatant colorimetrically at 540 nm using the 3,5-dinitrosalicylic acid (DNS) assay, modified by King et al (2008). In comparison to control cells, the mix of cellulase expressing P. putida cells show EFB degradation. Using glucose as standard the amount of released sugar equivalents (RSE) from from EFB after 4 days is 6.4 mg/ml (FIG. 37).

REFERENCES

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The invention claimed is:
 1. A method for displaying a recombinant polypeptide on the surface of a host cell, said method comprising the steps: (a) providing a host cell transformed with a nucleic acid fusion operatively linked with an expression control sequence, said nucleic acid fusion comprising: (i) a portion encoding a signal peptide allowing for transport into the periplasm through the inner cell membrane, (ii) a portion encoding the recombinant polypeptide to be displayed, (iii) a portion encoding a transmembrane linker, and (iv) a portion encoding the transporter domain of an EhaA protein, and (b) culturing the host cell under conditions wherein the nucleic acid fusion is expressed and the expression product comprising the recombinant polypeptide is displayed on the surface of the host cell, wherein the host cell is a Gram negative bacterium, with the proviso that the Gram negative bacterium is not E coli, wherein the transporter domain of the EhaA protein is encoded by a sequence comprising a sequence selected from the group consisting of: (1) a nucleotide sequence comprising SEQ ID NO:18, (2) a nucleotide sequence encoding SEQ ID NO:19, (3) nucleotide sequences comprising a sequence being at least 95% identical to SEQ ID NO:18 or/and a sequence encoding SEQ ID NO: 19, and (4) nucleotide sequences which encode the polypeptides encoded by (1), (2) or/and (3) within the scope of the degeneracy of the genetic code; wherein the transmembrane linker is encoded by a sequence comprising a sequence selected from the group consisting of (I) a nucleotide sequence comprising SEQ ID NO:16, (II) a nucleotide sequence encoding SEQ ID NO:17, (III) nucleotide sequences comprising a sequence being at least 95% identical to SEQ ID NO:16 or/and a sequence encoding SEQ ID NO: 17, and (IV) nucleotide sequences which encodes the polypeptides encoded by (I), (II) or/and (III) within the scope of the degeneracy of the genetic code.
 2. The method of claim 1, wherein the nucleic acid fusion further comprises at least one nucleic acid sequence encoding an affinity tag.
 3. The method of claim 2, wherein a nucleic acid sequence defined in claim 2 is flanking the portion (ii) encoding the recombinant polypeptide to be displayed.
 4. The method of claim 3, wherein the nucleic acid sequence is separated from portion (ii) by a sequence encoding at least one protease recognition sequence.
 5. The method of claim 4, wherein the at least one protease recognition sequence is independently selected from factor Xa cleavage site, OmpT cleavage site, and TEV protease cleavage site.
 6. The method of claim 2, wherein the affinity tag is independently selected from His₆ and epitopes.
 7. The method of claim 1, wherein the nucleic acid fusion comprises a nucleotide sequence encoding at least one protease recognition sequence, said nucleotide sequence being located between portions (ii) and portion (iii).
 8. The method of claim 7, wherein the at least one protease recognition sequence is independently selected from factor Xa cleavage site, OmpT cleavage site, and TEV protease cleavage site.
 9. The method of claim 1, wherein the portion (ii) encoding the recombinant polypeptide to be displayed is flanked by at least one sequence comprising a multiple cloning site.
 10. The method according to claim 1 wherein the transporter domain of the EhaA protein forms a β-barrel structure.
 11. The method of claim 1, wherein the transmembrane linker (ii) comprises a sequence selected from the group consisting of: (a) an amino acid sequence comprising SEQ ID NO:17, and (b) sequences which are at least 95% identical to the sequences of (a).
 12. The method of claim 1, wherein the transporter domain of the EhaA protein (iii) comprises a sequence selected from the group consisting of: (a) an amino acid sequence comprising SEQ ID NO:19, and (b) sequences which are at least 95% identical to the sequence of (a).
 13. The method of claim 1, wherein the sequence of the nucleic acid fusion has a codon usage adapted to the host cell.
 14. The method of claim 1, wherein the amino acid sequences encoded by nucleic acid sequences (i) to (iv) are arranged from N terminal to C terminal.
 15. The method of claim 1, wherein the nucleic acid sequences (i) to (iv) are arranged from 5′ to 3′.
 16. The method of claim 1, wherein the transporter domain of an EhaA protein is heterologous with respect to the host cell.
 17. The method of claim 1, wherein the host cell is a bacterium.
 18. The method of claim 1, wherein the bacterium is a Gram negative bacterium.
 19. The method of claim 1, wherein the bacterium is selected from Salmonella spp., Zymomonas spp., Zymobacter spp., Pseudomonas spp., Cupriavidus spp., Rhodobacter spp., Acinetobacter spp., Gluconobacter spp., Gluconacetobacter spp., Acidomonas spp., Acetobacter spp., Paracoccous spp., Rhizobium spp., and Xanthomonas spp.
 20. A recombinant vector comprising the nucleic acid fusion as defined in claim 1, operatively linked to an expression control sequence.
 21. A recombinant vector comprising: (i) a portion encoding a signal peptide allowing for transport into the periplasm through the inner cell membrane, (ii) a portion encoding a multiple cloning site, (iii) a portion encoding a transmembrane linker, and (iv) a portion encoding the transporter domain of an EhaA protein, wherein the transporter domain of the EhaA protein is encoded by a sequence comprising a sequence selected from the group consisting of: (1) a nucleotide sequence comprising SEQ ID NO:18, (2) a nucleotide sequence encoding SEQ ID NO:19, (3) nucleotide sequences comprising a sequence being at least 95% identical to SEQ ID NO:18 or/and a sequence encoding SEQ ID NO: 19, and (4) nucleotide sequences which encode the polypeptides encoded by (1), (2) or/and (3) within the scope of the degeneracy of the genetic code, and wherein the transmembrane linker is encoded by a sequence comprising a sequence selected from the group consisting of: (I) a nucleotide sequence comprising SEQ ID NO:16, (II) a nucleotide sequence encoding SEQ ID NO:17, (III) nucleotide sequences comprising a sequence being at least 95% identical to SEQ ID NO:16 or/and a sequence encoding SEQ ID NO: 17, and (IV) nucleotide sequences which encodes the polypeptides encoded by (I), (II) or/and (III) within the scope of the degeneracy of the genetic code.
 22. The vector of claim 21, wherein the multiple cloning site is suitable for integration of a nucleic acid sequence encoding a recombinant polypeptide in frame with portions (i), (ii) and (iv).
 23. The vector of claim 21, wherein the nucleic acid sequences (i) to (iv) are arranged from 5′ to 3′.
 24. A recombinant host cell comprising the recombinant vector as claimed in claim
 21. 25. A method for producing a host cell capable of displaying a recombinant polypeptide on the surface, said method comprising the steps (a) providing a vector comprising: (i) a portion encoding a signal peptide allowing for transport into the periplasm through the inner cell membrane, (ii) a portion encoding a multiple cloning site, (iii) a portion encoding a transmembrane linker, and (iv) a portion encoding the transporter domain of an EhaA protein (b) inserting a sequence encoding the recombinant polypeptide to be displayed into the multiple cloning site (iv), and (c) performing the method of claim 1, wherein the transporter domain of the EhaA protein is encoded by a sequence comprising a sequence selected from the group consisting of: (1) a nucleotide sequence comprising SEQ ID NO:18, (2) a nucleotide sequence encoding SEQ ID NO:19, (3) nucleotide sequences comprising a sequence being at least 95% identical to SEQ ID NO:18 or/and a sequence encoding SEQ ID NO: 19, and (4) nucleotide sequences which encode the polypeptides encoded by (1), (2) or/and (3) within the scope of the degeneracy of the genetic code, and wherein the transmembrane linker is encoded by a sequence comprising a sequence selected from the group consisting of: (I) a nucleotide sequence comprising SEQ ID NO:16, (II) a nucleotide sequence encoding SEQ ID NO:17, (Ill) nucleotide sequences comprising a sequence being at least 95% identical to SEQ ID NO:16 or/and a sequence encoding SEQ ID NO: 17, and (IV) nucleotide sequences which encodes the polypeptides encoded by (I), (II) or/and (III) within the scope of the degeneracy of the genetic code.
 26. A method for displaying a recombinant polypeptide on the surface of a host cell, said method comprising the steps: (a) providing a host cell transformed with a nucleic acid fusion operatively linked with an expression control sequence, said nucleic acid fusion comprising: (i) a portion encoding a signal peptide allowing for transport into the periplasm through the inner cell membrane, (ii) a portion encoding the recombinant polypeptide to be displayed, (iii) a portion encoding a transmembrane linker, and (iv) a portion encoding the transporter domain of an EhaA protein, and (b) culturing the host cell under conditions wherein the nucleic acid fusion is expressed and the expression product comprising the recombinant polypeptide is displayed on the surface of the host cell, wherein the recombinant polypeptide to be displayed is selected from an endoglucanase, an exoglucanase and a β-glucosidase, and combinations thereof, wherein the host cell is a Gram negative bacterium, and wherein the transporter domain of the EhaA protein is encoded by a sequence comprising a sequence selected from the group consisting of: (1) a nucleotide sequence comprising SEQ ID NO:18, (2) a nucleotide sequence encoding SEQ ID NO:19, (3) nucleotide sequences comprising a sequence being at least 95% identical to SEQ ID NO:18 or/and a sequence encoding SEQ ID NO: 19, and (4) nucleotide sequences which encode the polypeptides encoded by (1), (2) or/and (3) within the scope of the degeneracy of the genetic code, and wherein the transmembrane linker is encoded by a sequence comprising a sequence selected from the group consisting of: (I) a nucleotide sequence comprising SEQ ID NO:16, (II) a nucleotide sequence encoding SEQ ID NO:17, (III) nucleotide sequences comprising a sequence being at least 95% identical to SEQ ID NO:16 or/and a sequence encoding SEQ ID NO: 17, and (IV) nucleotide sequences which encodes the polypeptides encoded by (I), (II) or/and (III) within the scope of the degeneracy of the genetic code.
 27. A method for displaying a recombinant polypeptide on the surface of a host cell according to claim 1, wherein the sequence of the nucleic acid fusion has a codon usage adapted to the host cell.
 28. A method for displaying a recombinant polypeptide on the surface of a host cellusing a Maximized Autotransporter Expression System, said method comprising: (a) providing a host cell transformed with a nucleic acid fusion operatively linked with an expression control sequence, said nucleic acid fusion comprising: (i) a portion encoding a signal peptide which is a cholera toxin B subunit signal peptide, (ii) a portion encoding the recombinant polypeptide to be displayed, wherein said recombinant polypeptide is selected from the group consisting of an endoglucanase, an exoglucanase and a β-glucosidase, (iii) a portion encoding a transmembrane linker, wherein said transmembrane linker is encoded by a sequence comprising a sequence selected from the group consisting of: (1) a nucleotide sequence comprising SEQ ID NO:16, (2) a nucleotide sequence encoding SEQ ID NO:17, (3) a nucleotide sequence comprising a sequence which is at least 95% identical to SEQ ID NO:16 and/or a sequence encoding SEQ ID NO: 17, and (4) nucleotide sequences which encode the polypeptides encoded by (1) or/and (2) within the scope of the degeneracy of the genetic code, (iv) a portion encoding the transporter domain of an EhaA protein, wherein the transporter domain of the EhaA protein is encoded by a sequence comprising a sequence selected from the group consisting of: (A) a nucleotide sequence comprising SEQ ID NO:18, (B) a nucleotide sequence encoding SEQ ID NO:19, (C) a nucleotide sequence comprising a sequence which is at least 95% identical to SEQ ID NO:18 and/or a sequence encoding SEQ ID NO: 19, and (D) a nucleotide sequence which encodes the polypeptides encoded by (A), (B) or/and (C) within the scope of the degeneracy of the genetic code, and (b) culturing the host cell under conditions wherein the nucleic acid fusion is expressed and the expression product comprising the recombinant polypeptide is displayed on the surface of the host cell, wherein the host cell is a Gram negative bacterium, with the proviso that the Gram negative bacterium is not E coli.
 29. The method according to claim 1, wherein said recombinant polypeptide is encoded by a nucleotide sequence selected from the group consisting of SEQ ID NO: 20, SEQ ID NO:21, SEQ ID NO: 22, SEQ ID NO:23, SEQ ID NO: 24, and SEQ ID NO:25.
 30. The method of claim 1, wherein the signal peptide is a CtxB signal peptide.
 31. The method of claim 1, wherein the signal peptide is obtained from a gram-negative bacterium. 